Biochemical analysis of Hyalomma dromedarii salivary glands and gut tissues using SR-FTIR micro-spectroscopy.

Autor: Hendawy SHM; Parasitology and Animal Diseases Department, Veterinary Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt. seham.hendawy@wsu.edu.; Tick and Tick-Borne Diseases Research Unit, Veterinary Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt. seham.hendawy@wsu.edu.; Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA. seham.hendawy@wsu.edu., Alzan HF; Parasitology and Animal Diseases Department, Veterinary Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.; Tick and Tick-Borne Diseases Research Unit, Veterinary Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.; Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA., Abdel-Ghany HSM; Parasitology and Animal Diseases Department, Veterinary Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt.; Tick and Tick-Borne Diseases Research Unit, Veterinary Research Institute, National Research Centre, 33 El Buhouth St., Dokki, Giza, 12622, Egypt., Suarez CE; Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, 99164-7040, USA.; Animal Disease Research Unit, United States Department of Agricultural-Agricultural Research Service, Pullman, WA, USA., Kamel G; SESAME Synchrotron (Synchrotron-light for Experimental Science and Applications in the Middle East), Allan, 19252, Jordan. gihan.kamel@sesame.org.jo.; Department of Physics, Faculty of Science, Helwan University, Cairo, Egypt. gihan.kamel@sesame.org.jo.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Apr 12; Vol. 14 (1), pp. 8515. Date of Electronic Publication: 2024 Apr 12.
DOI: 10.1038/s41598-024-59165-6
Abstrakt: Ticks are obligatory voracious blood feeders infesting diverse vertebrate hosts, that have a crucial role in the transmission of diverse pathogens that threaten human and animal health. The continuous emergence of tick-borne diseases due to combined worldwide climatic changes, human activities, and acaricide-resistant tick strains, necessitates the development of novel ameliorative tick control strategies such as vaccines. The synchrotron-based Fourier transform infrared micro-spectroscopy (SR-FTIR) is a bioanalytical microprobe capable of exploring the molecular chemistry within microstructures at a cellular or subcellular level and is considered as a nondestructive analytical approach for biological specimens. In this study, SR-FTIR analysis was able to explore a qualitative and semi-quantitative biochemical composition of gut and salivary glands of Hyalomma dromedarii (H. dromedarii) tick detecting differences in the biochemical composition of both tissues. A notable observation regarding Amide I secondary structure protein profile was the higher ratio of aggregated strands in salivary gland and beta turns in gut tissues. Regarding the lipid profile, there was a higher intensity of lipid regions in gut tissue when compared to salivary glands. This detailed information on the biochemical compositions of tick tissues could assist in selecting vaccine and/or control candidates. Altogether, these findings confirmed SR-FTIR spectroscopy as a tool for detecting differences in the biochemical composition of H. dromedarii salivary glands and gut tissues. This approach could potentially be extended to the analysis of other ticks that are vectors of important diseases such as babesiosis and theileriosis.
(© 2024. The Author(s).)
Databáze: MEDLINE
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