The pFSH dose affects the efficiency of in vivo embryo production in Santa Inês ewes.

Autor: Ribeiro ACS; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil. Electronic address: ac_sarzedas@id.uff.br., Pinto PHN; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil., Santos JDR; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil., Ramalho NP; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil., Laeber CCR; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil., Balaro MFA; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil., Batista RITP; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil., Souza-Fabjan JMG; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil., da Fonseca JF; Embrapa Caprinos e Ovinos, Núcleo Regional Sudeste, Rodovia MG 133, Km 42, Coronel Pacheco, MG CEP 36155-000, Brazil., Ungerfeld R; Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Ruta 8 km 18, Montevideo 13000, Uruguay., Brandão FZ; Faculdade de Veterinária, Universidade Federal Fluminense, Av. Vital Brasil Filho, 64, Niterói, RJ CEP 24230-340, Brazil. Electronic address: fzbrandao@id.uff.br.
Jazyk: angličtina
Zdroj: Animal reproduction science [Anim Reprod Sci] 2024 May; Vol. 264, pp. 107459. Date of Electronic Publication: 2024 Mar 27.
DOI: 10.1016/j.anireprosci.2024.107459
Abstrakt: This study compared the follicular growth, superovulatory response, and in vivo embryo production after administering two doses of porcine follicle-stimulating hormone (pFSH) in Santa Inês ewes. The estrous cycle of 36 multiparous ewes was synchronized with the Day 0 protocol and superovulated with 133 mg (G133, n=18) or 200 mg (G200, n=18) of pFSH. Ultrasonographic evaluations of the ovaries were performed, ewes were mated and submitted to non-surgical embryo recovery. Viable blastocysts were stained with Nile Red and Hoechst. The G200 had a greater number of medium and large follicles, as well as a larger size of the third largest follicle. A total of 97.2% (35/36) of the ewes came into estrus and it was possible to transpose cervix in 80.6% (29/36). There were no effects of treatments in the response to superovulation, the proportion of ewes in which was possible to transpose the cervix, the number of corpora lutea, the number of anovulatory follicles, the proportion of ewes flushed with at least one recovered structure, number of recovered structures, number of viable embryos, viability rate, and recovery rate. The G200 ewes were in estrus for a longer period of time than the G133 ewes (54.0 ± 4.5 h vs. 40.3 ± 3.6 h) and produced more freezable embryos (6.5 ± 1.6 vs. 2.3 ± 0.7) than G133. Both doses promoted an efficient superovulatory response and did not affect embryonic lipid accumulation. The dose of 200 mg of pFSH showed greater potential to increase the superovulatory response, as it increased follicular recruitment and the recovery of freezable embryos.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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