EUS-guided biopsies versus surgical specimens for establishing patient-derived pancreatic cancer organoids: a systematic review and meta-analysis.

Autor: Grützmeier SE; Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, Herlev, Denmark. Electronic address: simon.ezban.gruetzmeier.01@regionh.dk., Sodal HMM; Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, Herlev, Denmark., Kovacevic B; Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, Herlev, Denmark; Department of Surgery and Transplantation, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark., Vilmann P; Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, Herlev, Denmark; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark., Karstensen JG; Department of Clinical Medicine, University of Copenhagen, Copenhagen, Denmark; Pancreatitis Centre East, Gastro Unit, Copenhagen University Hospital Amager and Hvidovre, Hvidovre, Denmark., Klausen P; Gastro Unit, Endoscopic Division, Copenhagen University Hospital Herlev and Gentofte, Herlev, Denmark; Department of Pathology, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
Jazyk: angličtina
Zdroj: Gastrointestinal endoscopy [Gastrointest Endosc] 2024 Oct; Vol. 100 (4), pp. 750-755. Date of Electronic Publication: 2024 Apr 07.
DOI: 10.1016/j.gie.2024.04.012
Abstrakt: Background and Aims: Patient-derived tumor organoids (PDTOs) are a promising new disease model in pancreatic cancer for use in personalized medicine. However, the overall success rate (SR) of establishing these cultures from EUS-guided biopsies is unknown.
Methods: We searched relevant database publications reporting SRs of PDTO establishment from pancreatic cancer. The primary outcome was SR stratified on tissue acquisition method (EUS-guided biopsies, percutaneous biopsies, and surgical specimens).
Results: Twenty-four studies were identified that included 1053 attempts at establishing PDTOs. Overall SR was 63% (95% confidence interval [CI], 54%-72%). Pooled SRs of PDTO establishment from EUS-guided biopsies, percutaneous biopsies, and surgical specimens were 60% (95% CI, 43%-76%), 36% (95% CI, 14%-61%), and 62% (95% CI, 48%-75%), respectively, and did not differ significantly (P = .1975).
Conclusion: The SR of PDTO establishment from EUS-guided biopsies is comparable to that from surgical specimens. Both techniques are suitable for tissue acquisition for PDTOs in clinical and research settings. (PROSPERO registration number: CRD42023425121.).
Competing Interests: Disclosure The following authors disclosed financial relationships: J. G. Karstensen: consultant for SNIPR BIOME, Boston Scientific, and Ambu. P. Vilmann: consultant for Medi-Globe GmbH. All other authors disclosed no financial relationships. This research was funded by the Danish Cancer Society (grant no. 2021 R322-A17350); the European Research Executive Agency under the project Training in Translational Protocols for Minimal Invasive Diagnosis and Therapy in Pancreatico-Biliary Cancers—TRIP (grant agreement no. 101079210); and Arvid Nilssons Fond, Tømrermester Jørgen Holm og hustru, Elisa f. Hansens Mindelegat, Dagmar Marshalls Fond, and Lægeforeningens Forskningsfond.
(Copyright © 2024 American Society for Gastrointestinal Endoscopy. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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