| Autor: |
Druzian DM; Applied Nanomaterials Research Group (GPNAp), Nanoscience Graduate Program, Franciscan University (UFN), Santa Maria, Rio Grande do Sul 97010-49, Brazil., Bonazza GKC; Cell Culture Laboratory and Bioactive Effects (LABCULTBIO), Nanoscience Graduate Program, Franciscan University (UFN), Santa Maria, Rio Grande do Sul 97010-491, Brazil., Sangoi GG; Cell Culture Laboratory and Bioactive Effects (LABCULTBIO), Nanoscience Graduate Program, Franciscan University (UFN), Santa Maria, Rio Grande do Sul 97010-491, Brazil., Machado AK; Cell Culture Laboratory and Bioactive Effects (LABCULTBIO), Nanoscience Graduate Program, Franciscan University (UFN), Santa Maria, Rio Grande do Sul 97010-491, Brazil., Moreno Ruiz YP; Academic Center of Vitoria (CAV), Department of Fundamental Chemistry (DQF), Federal University of Pernambuco (UFPE), Recife, State of Pernambuco 50740-560, Brazil., Galembeck A; Academic Center of Vitoria (CAV), Department of Fundamental Chemistry (DQF), Federal University of Pernambuco (UFPE), Recife, State of Pernambuco 50740-560, Brazil., Pavoski G; Polytechnical School of Chemical Engineering, University of the Sao Paulo (USP), São Paulo, State of São Paulo 05508-010, Brazil.; Department of Materials Engineering, The University of British Columbia, Vancouver Campus, British Columbia V6T 1Z4, Canada., Romano Espinosa DC; Polytechnical School of Chemical Engineering, University of the Sao Paulo (USP), São Paulo, State of São Paulo 05508-010, Brazil., da Silva WL; Applied Nanomaterials Research Group (GPNAp), Nanoscience Graduate Program, Franciscan University (UFN), Santa Maria, Rio Grande do Sul 97010-49, Brazil. |
| Abstrakt: |
Nowadays, bone systems have a series of consequences that compromise the quality of life mainly due to wear and decreased bioactivity, generally in elderly people and children. In this context, the combination of montmorillonite (MMT-NPs) in a vitreous system such as nanobioglass facilitates the adsorption of biomolecules on the surface and within the interlamellar spaces, enabling the entry of ions by a cation exchange process focusing on increasing the rate of bone formation. This work aims to synthesize and characterize an eco-friendly hybrid reinforcement containing MMT-NPs with nanobioglass doped with magnesium nanoparticles (MgNPs-BV). In this way, MMT-NPs@MgNPs-BV was synthesized by the impregnation method, where an experimental design was used to verify the synthesis conditions. The ideal condition by experimental design was carried out in terms of the characterization and biological activity, where we demonstrated MMT-NPs of 30% w w -1 , MgNPs-BV of 6% w w -1 , and a calcination temperature of 1273.15 K with a cell viability around 66.87%, an average crystallite diameter of 12.5 nm, and a contact angle of 17.7°. The characterizations confirmed the impregnation method with an average particle size of 51.4 ± 13.1 nm. The mechanical tests showed a hardness of 2.6 GPa with an apparent porosity of 22.2%, similar to human bone. MMT-NPs@MgNPs-BV showed a cell proliferation of around 96% in osteoblastic cells (OFCOL II), with the formation of the apatite phase containing a relation of Ca/P of around 1.63, a biodegradability of 82%, and rapid release of ions with a Ca/P ratio of 1.42. Therefore, the eco-friendly hybrid reinforcement with MMT-NPs and MgNPs-BV shows potential for application with a matrix for biocompatible nanocomposites for bone regeneration. |
