| Autor: |
Bhagavan H; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, United States., Wei AD; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, United States., Oliveira LM; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, United States., Aldinger KA; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, United States.; Department of Pediatrics, University of Washington, Seattle, Washington, United States.; Department of Neurology, University of Washington, Seattle, Washington, United States., Ramirez JM; Center for Integrative Brain Research, Seattle Children's Research Institute, Seattle, Washington, United States.; Department of Pediatrics, University of Washington, Seattle, Washington, United States.; Department of Neurological Surgery, University of Washington, Seattle, Washington, United States. |
| Jazyk: |
angličtina |
| Zdroj: |
American journal of physiology. Lung cellular and molecular physiology [Am J Physiol Lung Cell Mol Physiol] 2024 Jun 01; Vol. 326 (6), pp. L698-L712. Date of Electronic Publication: 2024 Apr 09. |
| DOI: |
10.1152/ajplung.00320.2023 |
| Abstrakt: |
Chronic intermittent hypoxia (CIH) is a prevalent condition characterized by recurrent episodes of oxygen deprivation, linked to respiratory and neurological disorders. Prolonged CIH is known to have adverse effects, including endothelial dysfunction, chronic inflammation, oxidative stress, and impaired neuronal function. These factors can contribute to serious comorbidities, including metabolic disorders and cardiovascular diseases. To investigate the molecular impact of CIH, we examined male C57BL/6J mice exposed to CIH for 21 days, comparing with normoxic controls. We used single-nucleus RNA sequencing to comprehensively examine the transcriptomic impact of CIH on key cell classes within the brainstem, specifically excitatory neurons, inhibitory neurons, and oligodendrocytes. These cell classes regulate essential physiological functions, including autonomic tone, cardiovascular control, and respiration. Through analysis of 10,995 nuclei isolated from pontine-medullary tissue, we identified seven major cell classes, further subdivided into 24 clusters. Our findings among these cell classes, revealed significant differential gene expression, underscoring their distinct responses to CIH. Notably, neurons exhibited transcriptional dysregulation of genes associated with synaptic transmission, and structural remodeling. In addition, we found dysregulated genes encoding ion channels and inflammatory response. Concurrently, oligodendrocytes exhibited dysregulated genes associated with oxidative phosphorylation and oxidative stress. Utilizing CellChat network analysis, we uncovered CIH-dependent altered patterns of diffusible intercellular signaling. These insights offer a comprehensive transcriptomic cellular atlas of the pons-medulla and provide a fundamental resource for the analysis of molecular adaptations triggered by CIH. NEW & NOTEWORTHY This study on chronic intermittent hypoxia (CIH) from pons-medulla provides initial insights into the molecular effects on excitatory neurons, inhibitory neurons, and oligodendrocytes, highlighting our unbiased approach, in comparison with earlier studies focusing on single target genes. Our findings reveal that CIH affects cell classes distinctly, and the dysregulated genes in distinct cell classes are associated with synaptic transmission, ion channels, inflammation, oxidative stress, and intercellular signaling, advancing our understanding of CIH-induced molecular responses. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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