Development of monoclonal antibodies against oropouche virus and its applicability to immunohistochemical diagnosis.

Autor: Andreolla AP; Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz PR, Cidade Industrial de Curitiba, Rua Prof. Algacyr Munhoz Mader 3775, Curitiba, Paraná, Brazil., Borges AA; Laboratório de Pesquisas em Virologia e Imunologia, Instituto de Ciências Biológicas e da Saúde (ICBS), Universidade Federal de Alagoas (UFAL), Av. Lourival Melo Mota, s/n, Tabuleiro do Martins, Maceió, Alagoas, Brazil., Nagashima S; Laboratório de Patologia Experimental, Pontifica Universidade Católica do Paraná (PUC/PR), Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná, Brazil., Vaz de Paula CB; Laboratório de Patologia Experimental, Pontifica Universidade Católica do Paraná (PUC/PR), Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná, Brazil., de Noronha L; Laboratório de Patologia Experimental, Pontifica Universidade Católica do Paraná (PUC/PR), Rua Imaculada Conceição, 1155, Prado Velho, Curitiba, Paraná, Brazil., Zanchin NIT; Laboratório de Biologia Estrutural e Engenharia de Proteínas, Instituto Carlos Chagas, ICC/Fiocruz PR, Cidade Industrial de Curitiba, Rua Prof. Algacyr Munhoz Mader 3775, Curitiba, Paraná, Brazil., Bordignon J; Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz PR, Cidade Industrial de Curitiba, Rua Prof. Algacyr Munhoz Mader 3775, Curitiba, Paraná, Brazil. juliano.bordignon@fiocruz.br., Duarte Dos Santos CN; Laboratório de Virologia Molecular, Instituto Carlos Chagas, ICC/Fiocruz PR, Cidade Industrial de Curitiba, Rua Prof. Algacyr Munhoz Mader 3775, Curitiba, Paraná, Brazil. claudia.dossantos@fiocruz.br.
Jazyk: angličtina
Zdroj: Virology journal [Virol J] 2024 Apr 08; Vol. 21 (1), pp. 81. Date of Electronic Publication: 2024 Apr 08.
DOI: 10.1186/s12985-024-02323-z
Abstrakt: Orthobunyavirus oropouche ense virus (OROV), the causative agent of Oropouche fever, is widely dispersed in Brazil and South America, causing sporadic outbreaks. Due to the similarity of initial clinical symptoms caused by OROV with other arboviruses found in overlapping geographical areas, differential diagnosis is challenging. As for most neglected tropical diseases, there is a shortage of reagents for diagnosing and studying OROV pathogenesis. We therefore developed and characterized mouse monoclonal antibodies and, one of them recognizes the OROV nucleocapsid in indirect immunofluorescent (IFA) and immunohistochemistry (IHC) assays. Considering that it is the first monoclonal antibody produced for detecting OROV infections, we believe that it will be useful not only for diagnostic purposes but also for performing serological surveys and epidemiological surveillance on the dispersion and prevalence of OROV in Brazil and South America.
(© 2024. The Author(s).)
Databáze: MEDLINE
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