Effects of sporadic inclusion body myositis on skeletal muscle fibre type specific morphology and markers of regeneration and inflammation.

Autor: Jensen KY; Copenhagen Research Center for Autoimmune Connective Tissue Diseases (COPEACT), Center for Rheumatology and Spine Diseases, Copenhagen University Hospital, Rigshospitalet, Juliane Maries Vej 10, 2100, Copenhagen, Denmark. Kasper.yde.jensen@regionh.dk.; Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark. Kasper.yde.jensen@regionh.dk.; Department of Pathology, Department of Clinical Research, University of Southern Denmark, Odense University Hospital, Odense, Denmark. Kasper.yde.jensen@regionh.dk., Nielsen JL; Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark., Aagaard P; Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark., Jacobsen M; Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark.; Department of Pathology, Department of Clinical Research, University of Southern Denmark, Odense University Hospital, Odense, Denmark., Jørgensen AN; Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark.; Department of Clinical Research, University of Southern Denmark, Odense, Denmark., Bech RD; Department of Orthopaedics and Traumatology, Zealand University Hospital, Koege, Denmark., Frandsen U; Department of Sports Science and Clinical Biomechanics, University of Southern Denmark, Odense, Denmark., Diederichsen LP; Copenhagen Research Center for Autoimmune Connective Tissue Diseases (COPEACT), Center for Rheumatology and Spine Diseases, Copenhagen University Hospital, Rigshospitalet, Juliane Maries Vej 10, 2100, Copenhagen, Denmark.; Department of Rheumatology, Odense University Hospital, Odense, Denmark., Schrøder HD; Department of Pathology, Department of Clinical Research, University of Southern Denmark, Odense University Hospital, Odense, Denmark.
Jazyk: angličtina
Zdroj: Rheumatology international [Rheumatol Int] 2024 Jun; Vol. 44 (6), pp. 1077-1087. Date of Electronic Publication: 2024 Apr 06.
DOI: 10.1007/s00296-024-05567-8
Abstrakt: Sporadic inclusion body myositis (sIBM) is a subgroup of idiopathic inflammatory myopathies characterised by progressive muscle weakness and skeletal muscle inflammation. Quantitative data on the myofibre morphology in sIBM remains scarce. Further, no previous study has examined fibre type association of satellite cells (SC), myonuclei number, macrophages, capillaries, and myonuclear domain (MD) in sIBM patients. Muscle biopsies from sIBM patients (n = 18) obtained previously (NCT02317094) were included in the analysis for fibre type-specific myofibre cross-sectional area (mCSA), SCs, myonuclei and macrophages, myonuclear domain, and capillarisation. mCSA (p < 0.001), peripheral myonuclei (p < 0.001) and MD (p = 0.005) were higher in association with type 1 (slow-twitch) than type 2 (fast-twitch) fibres. Conversely, quiescent SCs (p < 0.001), centrally placed myonuclei (p = 0.03), M1 macrophages (p < 0.002), M2 macrophages (p = 0.013) and capillaries (p < 0.001) were higher at type 2 fibres compared to type 1 fibres. In contrast, proliferating (Pax7 + /Ki67 + ) SCs (p = 0.68) were similarly associated with each fibre type. Type 2 myofibres of late-phase sIBM patients showed marked signs of muscle atrophy (i.e. reduced mCSA) accompanied by higher numbers of associated quiescent SCs, centrally placed myonuclei, macrophages and capillaries compared to type 1 fibres. In contrast, type 1 fibres were suffering from pathological enlargement with larger MDs as well as fewer nuclei and capillaries per area when compared with type 2 fibres. More research is needed to examine to which extent different therapeutic interventions including targeted exercise might alleviate these fibre type-specific characteristics and countermeasure their consequences in impaired functional performance.
(© 2024. The Author(s).)
Databáze: MEDLINE
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