Fructose improves titanium dioxide nanoparticles induced alterations in developmental competence of mouse oocytes.

Autor: Elmetwally MA; Department of Theriogenology, Center for Reproductive Biotechnology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt. mmetwally@mans.edu.eg., Helmy A; Fertility care center, Obstetrics and Gynecology department, faculty of medicine, Mansoura University, Mansoura, 35516, Egypt., Balboula A; Department of animal science, University of Missouri, Columbia, MO, 65211, USA.; Center for Reproductive Biotechnology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Eladl M; Department of Biochemistry and Molecular Biology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Hamed B; Medical research center, faculty of medicine, Mansoura University, Mansoura, 35516, Egypt., Lashen S; Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Rezk S; Department of Cytology and Histology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Yaseen A; Fertility care center, Obstetrics and Gynecology department, faculty of medicine, Mansoura University, Mansoura, 35516, Egypt., Sharawy H; Department of Theriogenology, Center for Reproductive Biotechnology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Hussien M; Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Zabel S; Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Montaser A; Department of Theriogenology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt., Halawa A; Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
Jazyk: angličtina
Zdroj: BMC veterinary research [BMC Vet Res] 2024 Apr 03; Vol. 20 (1), pp. 135. Date of Electronic Publication: 2024 Apr 03.
DOI: 10.1186/s12917-024-03963-7
Abstrakt: Aims: We investigated the effects of intraperitoneal injections of titanium dioxide nanoparticles (TiO 2 NPs, 100 mg/kg) for 5 consecutive days on the developmental competence of murine oocytes. Furthermore, study the effects of TiO 2 NPs on antioxidant and oxidative stress biomarkers, as well as their effects on expression of apoptotic and hypoxia inducing factor-1α (HIF1A) protein translation. Moreover, the possible ameliorating effects of intraperitoneal injections of fructose (2.75 mM/ml) was examined.
Materials and Methods: Thirty sexually mature (8-12 weeks old; ~ 25 g body weight) female mice were used for the current study. The female mice were assigned randomly to three treatment groups: Group1 (G1) mice were injected intraperitoneal (ip) with deionized water for 5 consecutive days; Group 2 (G2) mice were injected ip with TiO 2 NPs (100 mg/kg BW) for 5 consecutive days; Group 3 (G3) mice were injected ip with TiO 2 NPs (100 mg/kg BW + fructose (2.75 mM) for 5 consecutive days.
Results: Nano-titanium significantly decreased expression of GSH, GPx, and NO, expression of MDA and TAC increased. The rates of MI, MII, GVBD and degenerated oocytes were significantly less for nano-titanium treated mice, but the rate of activated oocytes was significantly greater than those in control oocytes. TiO 2 NPs significantly increased expression of apoptotic genes (BAX, Caspase 3 and P53) and HIF1A. Intraperitoneal injection of fructose (2.75 mM/kg) significantly alleviated the detrimental effects of TiO 2 NPs. Transmission electron microscopy indicated that fructose mitigated adverse effects of TiO2 NPs to alter the cell surface of murine oocytes.
Conclusion: Results of this study suggest that the i/p infusion of fructose for consecutive 5 days enhances development of murine oocytes and decreases toxic effects of TiO 2 NPs through positive effects on oxidative and antioxidant biomarkers in cumulus-oocyte complexes and effects to inhibit TiO2-induced increases in expression of apoptotic and hypoxia inducing factors.
(© 2024. The Author(s).)
Databáze: MEDLINE
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