Development of a corn flour certified reference material for the accurate determination of zearalenone.

Autor: Lijalem YG; Organic Metrology Group, Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Daejeon, 34113, South Korea.; Department of Bio-Analytical Science, University of Science and Technology, Daejeon, 34113, South Korea.; National Metrology Institute of Ethiopia, P. O. Box: 5722, Addis Ababa, Ethiopia., Gab-Allah MA; Organic Metrology Group, Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Daejeon, 34113, South Korea.; Department of Bio-Analytical Science, University of Science and Technology, Daejeon, 34113, South Korea.; Reference Materials Lab, National Institute of Standards, Tersa St, Haram, P. O. Box: 136, Giza, 12211, Egypt., Yu H; Organic Metrology Group, Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Daejeon, 34113, South Korea.; Department of Bio-Analytical Science, University of Science and Technology, Daejeon, 34113, South Korea., Choi K; Organic Metrology Group, Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Daejeon, 34113, South Korea. kihwan.choi@kriss.re.kr.; Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, 34134, South Korea. kihwan.choi@kriss.re.kr., Kim B; Organic Metrology Group, Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science, Daejeon, 34113, South Korea. byungjoo@kriss.re.kr.; Department of Bio-Analytical Science, University of Science and Technology, Daejeon, 34113, South Korea. byungjoo@kriss.re.kr.
Jazyk: angličtina
Zdroj: Analytical and bioanalytical chemistry [Anal Bioanal Chem] 2024 May; Vol. 416 (13), pp. 3173-3183. Date of Electronic Publication: 2024 Apr 03.
DOI: 10.1007/s00216-024-05265-1
Abstrakt: A certified reference material (CRM, KRISS 108-01-002) for zearalenone in corn flour was developed to assure reliable and accurate measurements in testing laboratories. Commercially available corn flour underwent freeze-drying, pulverization, sieving, and homogenization. The final product was packed in amber bottles, approximately 14 g per unit, and preserved at -70 °C. 13 C 18 -Zearalenone was used as an internal standard (IS) for the certification of zearalenone by isotope-dilution liquid chromatography-tandem mass spectrometry (ID-LC‒MS/MS) and for the analysis of α-zearalenol, β-zearalenol, and zearalanone by LC‒MS/MS. The prepared CRM was sufficiently homogeneous, as the among-unit relative standard deviation for each mycotoxin ranged from 2.2 to 5.7 %. Additionally, the stability of the mycotoxins in the CRM was evaluated under different temperature conditions and scheduled test periods, including storage at -70°C, -20°C, and 4°C and room temperature for up to 12 months, 6 months, and 1 month, respectively. The content of each target mycotoxin in the CRM remained stable throughout the monitoring period at each temperature. Zearalenone content (153.6 ± 8.0 µg/kg) was assigned as the certified value. Meanwhile, the contents of α-zearalenol (1.30 ± 0.17 µg/kg), β-zearalenol (4.75 ± 0.33 µg/kg), and zearalanone (2.09 ± 0.16 µg/kg) were provided as informative values.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
Databáze: MEDLINE
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