Establishment of cluster of differentiation 20 immobilized cell membrane chromatography for the screening of active antitumor components in traditional Chinese medicine.

Autor: Ding Y; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China., Guo N; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China., Jiang Y; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China., Liu S; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China., Zhou T; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China., Bai H; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China., Lv Y; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China., Han S; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China. Electronic address: slhan2008@mail.xjtu.edu.cn., He L; School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an 710061, PR China; Institute of Pharmaceutical Science and Technology, Western China Science &Technology Innovation Harbour, Xi'an 710115, PR China. Electronic address: helc@mail.xjtu.edu.cn.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Apr 26; Vol. 1721, pp. 464845. Date of Electronic Publication: 2024 Mar 24.
DOI: 10.1016/j.chroma.2024.464845
Abstrakt: Non-Hodgkin lymphoma (NHL) is a heterogeneous group of malignant tumors occurring in B or T lymphocytes, and no small molecule-positive drugs to treat NHL have been marketed. Cluster of differentiation 20 (CD20) is an important molecule regulating signaling for the life and differentiation of B lymphocytes and possesses the characteristics of a drug target for treating NHL. 2-Methoxyestradiol induces apoptosis in lymphoma Raji cells and CD20 protein is highly expressed by Raji lymphoma cells. Therefore, in this study, a CD20-SNAP-tag/CMC model was developed to validate the interaction of 2-methoxyestradiol with CD20. 2-Methoxyestradiol was used as a small molecule control compound, and the system was validated for good applicability. The cell membrane chromatography model was combined with high-performance liquid chromatography ion trap time-of-flight mass spectroscopy (HPLC-IT-TOF-MS) in a two-dimensional system to successfully identify, analyze, and characterize the potential active compounds of Schisandra chinensis (Turcz.) Baill. extract and Lysionotus pauciflorus Maxim. extract, including Schisandrin A, Schizandrol A, Schizandrol B, Schisantherin B, and Nevadensin, which can act on CD20 receptors. The five potential active compounds were analyzed by non-linear chromatography. The thermodynamic and kinetic parameters of their interaction with CD20 were also analyzed, and the mode of interaction was simulated by molecular docking. Their inhibitory effects on lymphoma cell growth were assessed using a Cell Counting Kit-8 (CCK-8). Nevadensin and Schizandrin A were able to induce apoptosis in Raji cells within a certain concentration range. In conclusion, the present experiments provide some bases for improving NHL treatment and developing small molecule lead compounds targeting CD20 with low toxicity and high specificity.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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