| Autor: |
García-Cambrón JB; Programa de Doctorado en Biología Experimental, Universidad Autónoma Metropolitana, Iztapalapa, Ciudad de México 09089, Mexico., Cerriteño-Sánchez JL; Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Cuajimalpa, Ciudad de México 05110, Mexico., Lara-Romero R; Programa de Estancia Posdoctoral, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México, Ciudad de México 04510, Mexico., Quintanar-Guerrero D; División de Estudios de Posgrado (Tecnología Farmacéutica), Facultad de Estudios Superiores, Universidad Nacional Autónoma de México, Cuautitlán Izcalli, Estado de México 54740, Mexico., Blancas-Flores G; Laboratorio de Farmacología, División de Ciencias Biológicas y de la Salud, Universidad Autónoma Metropolitana, Iztapalapa, Ciudad de México 09089, Mexico., Sánchez-Gaytán BL; Centro de Química ICUAP, Laboratorio de Bioinorgánica Aplicada, Benemérita Universidad Autónoma de Puebla, Puebla 72592, Mexico., Herrera-Camacho I; Centro de Química ICUAP, Laboratorio de Bioquímica y Biología Molecular, Edificio IC7, Benemérita Universidad Autónoma de Puebla, Puebla 72592, Mexico., Cuevas-Romero JS; Instituto Nacional de Investigaciones Forestales Agrícolas y Pecuarias, Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Cuajimalpa, Ciudad de México 05110, Mexico. |
| Abstrakt: |
Porcine epidemic diarrhea virus (PEDV) has affected the pork industry worldwide and during outbreaks the mortality of piglets has reached 100%. Lipid nanocarriers are commonly used in the development of immunostimulatory particles due to their biocompatibility and slow-release delivery properties. In this study, we developed a lipid nanoparticle (LNP) complex based on glycyrrhizinic acid (GA) and tested its efficacy as an adjuvant in mice immunized with the recombinant N-terminal domain (NTD) of porcine epidemic diarrhea virus (PEDV) spike (S) protein (rNTD-S). The dispersion stability analysis (Z-potential -27.6 mV) confirmed the size and charge stability of the LNP-GA, demonstrating that the particles were homogeneously dispersed and strongly anionic, which favors nanoparticles binding with the rNTD-S protein, which showed a slightly positive charge (2.11 mV) by in silico analysis. TEM image of LNP-GA revealed nanostructures with a spherical-bilayer lipid vesicle (~100 nm). The immunogenicity of the LNP-GA-rNTD-S complex induced an efficient humoral response 14 days after the first immunization ( p < 0.05) as well as an influence on the cellular immune response by decreasing serum TNF-α and IL-1β concentrations, which was associated with an anti-inflammatory effect. |
