| Autor: |
Schettino MT; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Preti EP; Preventive Gynecology Unit, European Institute of Oncology, 20100 Milano, Italy., Vietri V; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Agrillo N; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Iavazzo N; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Fasulo DD; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., De Franciscis P; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Campitiello MR; ASL Salerno, 84100 Salerno, Italy., Vastarella MG; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Riemma G; Department of Woman, Child and General and Specialized Surgery, University of Campania 'Luigi Vanvitelli', 80138 Naples, Italy., Gardella B; Department of Obstetrics and Gynecology, Fondazione IRCCS Policlinico San Matteo, 27100 Pavia, Italy., Murina F; Lower Genital Tract Disease Unit, Obstetrics and Gynecology Department, V. Buzzi Hospital, University of Milan, 20154 Milan, Italy. |
| Abstrakt: |
Background and Objectives : Available evidence reports the overexpression of β1 integrin in dysplastic rather than normal cervical tissue. We aimed to evaluate the involvement of β1 (CD29) integrin in the progressive pathogenesis of cervical intraepithelial neoplasia (CIN). Materials and Methods : From January 2019 to December 2021, we prospectively enrolled women undergoing a colposcopy with a cervical biopsy for abnormal cervical cytology and/or undefined cytology with a positive HPV DNA test and women with relapsing cervical inflammatory disorders. Based on the histopathological results, women were divided into four groups: group A (CIN1), group B (CIN2), group C (CIN3), and group D (no CIN diagnosis) as a control group. Subsequently, cytofluorimetry and immunohistochemical analysis (based on the identified positive cell ratios as follows: ≤10%, negative; 10-25%, 1+ (weak); 25-50%, 2+ (medium); ≥50%, and 3+ (high)) for β1 integrin were carried out. Results : In total, 154 women were included. The average fluorescence intensity in the four groups was 2.35 ± 1.37, 2.73 ± 1.56, 3.09 ± 1.56, and 2.13 ± 1.25 UA from groups A to D, respectively; this figure was significantly different for CIN3 (group C) women relative to the other groups ( p = 0.0132). Higher β1 integrin/CD29 concentrations in the CIN groups with HR-HPV 16 and 18 were also detected ( p = 0.0292, 0.0367, and 0.0357 respectively for CIN3, CIN2, and CIN1). Immunohistochemistry analysis showed higher results for the CIN3 group compared to controls and all the other groups ( p < 0.001). Conclusions : β1/CD29 integrin expression increased with CIN grade, and it was significantly higher in CIN3 lesions. This could be used as a promising screening tool to identify women prone to developing high-grade cervical lesions. However, additional evidence is needed to strengthen these findings. |
