New Insights into Cutaneous Asepsis: Synergism between Pfaffia and Rosemary Extracts.

Autor: Miranda DG; Gynecology and Obstetrics Service, Woman-Mother-Child Hospital, Hospices Civils de Lyon, 59 Boulevard Pinel, 69500 Lyon, France.; Laboratory Health Systemic Process (P2S), UR4129, Faculty of Medicine Laenenc, University Claude Bernard Lyon 1, University of Lyon, 11 Rue Guillaume Paradin, 69008 Lyon, France.; Multimaterials and Interfaces Laboratory, CNRS (UMR 5615), University Claude Bernard Lyon 1, 6 Rue Victor Grignard, 69622 Lyon, France.; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil., Carrouel F; Laboratory Health Systemic Process (P2S), UR4129, Faculty of Medicine Laenenc, University Claude Bernard Lyon 1, University of Lyon, 11 Rue Guillaume Paradin, 69008 Lyon, France., Silva TCA; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil.; Department of Health Sciences, Paulista University, Highway President Dutra, São José dos Campos 12240-420, São Paulo, Brazil., Rozzatto MC; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil.; Department of Health Sciences, Paulista University, Highway President Dutra, São José dos Campos 12240-420, São Paulo, Brazil., Hasna AA; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil.; School of Dentistry, Espiritu Santo University, Avenue Samborondon 5, Samborondón 092301, Ecuador., Santos CER; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil., Morais FV; Department of Health Sciences, Paulista University, Highway President Dutra, São José dos Campos 12240-420, São Paulo, Brazil.; Laboratory of Cell and Molecular Biology of Fungi, Research and Development Institute, Paraíba Valley University, Avenue Shishima Hifumi 2911, São José dos Campos 12244-010, São Paulo, Brazil., de Oliveira LD; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil., de Paula Ramos L; Laboratory Health Systemic Process (P2S), UR4129, Faculty of Medicine Laenenc, University Claude Bernard Lyon 1, University of Lyon, 11 Rue Guillaume Paradin, 69008 Lyon, France.; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University, Francisco José Longo 777, São José dos Campos 12245-000, São Paulo, Brazil.; Department of Health Sciences, Paulista University, Highway President Dutra, São José dos Campos 12240-420, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Antibiotics (Basel, Switzerland) [Antibiotics (Basel)] 2024 Feb 28; Vol. 13 (3). Date of Electronic Publication: 2024 Feb 28.
DOI: 10.3390/antibiotics13030226
Abstrakt: (1) Background: In hospitals, medical and dental clinics, antiseptics or disinfectants play an essential role in the control of nosocomial infections. This study aimed to evaluate R. officinalis and P. paniculata glycolic extracts regarding: (I) their antimicrobial action on planktonic and biofilm (monotypic and cutaneous biofilm model- S. aureus , S. epidermidis and C. acnes ); and (II) their cytotoxicity on human keratinocytes (HaCaT). (2) Methods: Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were performed (CLSI protocol M7-A6 and M11-A8). MTT analysis was used to evaluate the antibiofilm activity of the extracts on biofilms and their cytotoxicity on human keratinocytes. (3) Results: The combined glycolic extracts MIX A (75% P. paniculata + 25% R. officinalis) ; MIX B (50% P. paniculata + 50% R. officinalis ); and MIX C (25% P. paniculata + 75% R. officinalis ) promoted MBC values by 50 mg/mL on S. aureus , absent on S. epidermidis , and ranged 6.25-50 mg/mL for C. acnes. The cutaneous biofilm model was reduced more than 90%. In addition, it showed biocompatibility with human keratinocytes, resulting in percentages of viability greater than 50%. (4) Conclusions: The combination of extracts promoted antimicrobial action on planktonic cultures, and monotypic and heterotypic biofilms of skin pathogens. Additionally, these extracts are biocompatible against human keratinocytes.
Databáze: MEDLINE