Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus.
| Autor: | Szczotka M; Department of Biochemistry, National Veterinary Research Institute, 24-100 Puławy, Poland., Kuźmak J; Department of Biochemistry, National Veterinary Research Institute, 24-100 Puławy, Poland. |
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| Jazyk: | angličtina |
| Zdroj: | Journal of veterinary research [J Vet Res] 2024 Mar 23; Vol. 68 (1), pp. 19-33. Date of Electronic Publication: 2024 Mar 23 (Print Publication: 2024). |
| DOI: | 10.2478/jvetres-2024-0012 |
| Abstrakt: | Introduction: Bovine leukaemia virus (BLV) is a Deltaretrovirus responsible for enzootic bovine leukosis, the most common neoplastic disease of cattle. It deregulates the immune system, favouring secondary infections and changes in the blood and lymphatic tissues. Blood homeostasis depends on functional haematopoietic stem cells (HSCs). Bone marrow is populated by these cells, which express CD34 + and CD35 + surface antigens and produce and release cytokines involved in the maintenance of haematopoiesis. The aim of the study was determination of the profile of cytokine production by CD34 + stem cells of cattle naturally infected with BLV. Material and Methods: The HSCs were generated from the blood and lymphoid organs of cows infected with BLV and healthy control cows with immunomagnetic separation and anti-CD34 + monoclonal antibodies. Isolated CD34 + cells were cultivated for two weeks with interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor. The levels of IL-6, IL-10, IL-12p40, IL-12p70, interferon gamma (IFN-γ) and tumour necrosis factor alpha (TNF-α) were determined in culture fluid by flow cytometry. Results: The expression of IL-6, IL-12p70 and TNF-α in blood HSCs was higher in BLV + cows than in the control animals. In bone marrow HSCs of infected cows, IL-12, TNF-α and IFN-γ were more concentrated, but in these cows' spleen HSCs only expression of IL-10 was elevated. In HSCs isolated from the lymph nodes of leukaemic cows, only TNF-α secretion was lower than in control cows, the other cytokines being more potently secreted. Conclusion: Infection with BLV caused statistically significant differences in cytokine expression by HSC CD34 + cells. Competing Interests: Conflict of Interests Statement: The authors declare that there is no conflict of interests regarding the publication of this article. (© 2024 Maria Szczotka et al., published by Sciendo.) |
| Databáze: | MEDLINE |
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