Genome-wide association study reveals SNP markers controlling drought tolerance and related agronomic traits in chickpea across multiple environments.

Autor:	Istanbuli T; Biotechnology Department, International Center for Agricultural Research in the Dry Areas (ICARDA), Terbol, Lebanon., Nassar AE; Biotechnology Department, International Center for Agricultural Research in the Dry Areas (ICARDA), Giza, Egypt., Abd El-Maksoud MM; Department of Genetics, Faculty of Agriculture, Mansoura University, Mansoura, Egypt., Tawkaz S; Biotechnology Department, International Center for Agricultural Research in the Dry Areas (ICARDA), Giza, Egypt., Alsamman AM; Biotechnology Department, International Center for Agricultural Research in the Dry Areas (ICARDA), Giza, Egypt.; Genome Mapping Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC), Giza, Egypt., Hamwieh A; Biotechnology Department, International Center for Agricultural Research in the Dry Areas (ICARDA), Giza, Egypt.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 Mar 08; Vol. 15, pp. 1260690. Date of Electronic Publication: 2024 Mar 08 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1260690
Abstrakt:	Chickpea, renowned for its exceptional nutritional value, stands as a crucial crop, serving as a dietary staple in various parts of the world. However, its productivity faces a significant challenge in the form of drought stress. This challenge highlights the urgent need to find genetic markers linked to drought tolerance for effective breeding programs. The primary objective of this study is to identify genetic markers associated with drought tolerance to facilitate effective breeding programs. To address this, we cultivated 185 chickpea accessions in two distinct locations in Lebanon over a two-year period, subjecting them to both irrigated and rain-fed environments. We assessed 11 drought-linked traits, including morphology, growth, yield, and tolerance score. SNP genotyping revealed 1344 variable SNP markers distributed across the chickpea genome. Genetic diversity across populations originating from diverse geographic locations was unveiled by the PCA, clustering, and structure analysis indicating that these genotypes have descend from five or four distinct ancestors. A genome-wide association study (GWAS) revealed several marker trait associations (MTAs) associated with the traits evaluated. Within the rainfed conditions, 11 significant markers were identified, each associated with distinct chickpea traits. Another set of 11 markers exhibited associations in both rainfed and irrigated environments, reflecting shared genetic determinants across these conditions for the same trait. The analysis of linkage disequilibrium (LD) highlighted two genomic regions with notably strong LD, suggesting significant interconnections among several investigated traits. This was further investigated by the correlation between major markers associated with these traits. Gene annotation of the identified markers has unveiled insights into 28 potential genes that play a role in influencing various chickpea drought-linked traits. These traits encompass crucial aspects such as blooming organ development, plant growth, seed weight, starch metabolism, drought regulation, and height index. Among the identified genes are CPN60-2 , hsp70 , GDSL(GELP) , AHL16 , NAT3 , FAB1B , bZIP , and GL21 . These genes collectively contribute to the multifaceted response of chickpea plants to drought stress. Our identified genetic factors exert their influence in both irrigated and rainfed environments, emphasizing their importance in shaping chickpea characteristics. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Istanbuli, Nassar, Abd El-Maksoud, Tawkaz, Alsamman and Hamwieh.)
Databáze:	MEDLINE
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