Genomic characterization of a multidrug-resistant uropathogenic Escherichia coli and evaluation of Echeveria plant extracts as antibacterials.

Autor:	Castañeda-Meléndrez AM; Unidad de Investigaciones en Salud Pública 'Dra. Kaethe Willms', Facultad de Ciencias Químico-Biológicas. Universidad Autónoma de Sinaloa. Ciudad Universitaria, Culiacán, Sinaloa, México., Magaña-Lizárraga JA; Unidad de Investigaciones en Salud Pública 'Dra. Kaethe Willms', Facultad de Ciencias Químico-Biológicas. Universidad Autónoma de Sinaloa. Ciudad Universitaria, Culiacán, Sinaloa, México., Martínez-Valenzuela M; Unidad de Investigaciones en Salud Pública 'Dra. Kaethe Willms', Facultad de Ciencias Químico-Biológicas. Universidad Autónoma de Sinaloa. Ciudad Universitaria, Culiacán, Sinaloa, México., Clemente-Soto AF; Unidad de Investigaciones en Salud Pública 'Dra. Kaethe Willms', Facultad de Ciencias Químico-Biológicas. Universidad Autónoma de Sinaloa. Ciudad Universitaria, Culiacán, Sinaloa, México., García-Cervantes PC; Unidad de Investigaciones en Salud Pública 'Dra. Kaethe Willms', Facultad de Ciencias Químico-Biológicas. Universidad Autónoma de Sinaloa. Ciudad Universitaria, Culiacán, Sinaloa, México., Delgado-Vargas F; Unidad de Investigaciones en Salud Pública 'Dra. Kaethe Willms', Facultad de Ciencias Químico-Biológicas. Universidad Autónoma de Sinaloa. Ciudad Universitaria, Culiacán, Sinaloa, México., Bernal-Reynaga R; Unidad de Investigaciones en Salud Pública 'Dra. Kaethe Willms', Facultad de Ciencias Químico-Biológicas. Universidad Autónoma de Sinaloa. Ciudad Universitaria, Culiacán, Sinaloa, México.
Jazyk:	angličtina
Zdroj:	AIMS microbiology [AIMS Microbiol] 2024 Jan 17; Vol. 10 (1), pp. 41-61. Date of Electronic Publication: 2024 Jan 17 (Print Publication: 2024).
DOI:	10.3934/microbiol.2024003
Abstrakt:	Uropathogenic Escherichia coli (UPEC) is the most common bacterial agent associated with urinary tract infections, threatening public health systems with elevated medical costs and high morbidity rates. The successful establishment of the infection is associated with virulence factors encoded in its genome, in addition to antibacterial resistance genes, which could limit the treatment and resolution of the infection. In this sense, plant extracts from the genus Echeveria have traditionally been used to treat diverse infectious diseases. However, little is known about the effects of these extracts on bacteria and their potential mechanisms of action. This study aims to sequence a multidrug-resistant UPEC isolate (UTI-U7) and assess the multilocus sequence typing (MLST), virulence factors, antimicrobial resistance profile, genes, serotype, and plasmid content. Antimicrobial susceptibility profiling was performed using the Kirby-Bauer disk diffusion. The antibacterial and anti-adherent effects of the methanol extracts (ME) of Echeveria ( E. craigiana , E. kimnachii , and E. subrigida ) against UTI-U7 were determined. The isolate was characterized as an O25:H4-B2-ST2279-CH40 subclone and had resistant determinants to aminoglycosides, β-lactams, fluoroquinolones/quinolones, amphenicols, and tetracyclines, which matched with the antimicrobial resistance profile. The virulence genes identified encode adherence factors, iron uptake, protectins/serum resistance, and toxins. Identified plasmids belonged to the IncF group (IncFIA, IncFIB, and IncFII), alongside several prophage-like elements. After an extensive genome analysis that confirmed the pathogenic status of UTI-U7 isolate, Echeveria extracts were tested to determine their antibacterial effects; as an extract, E. subrigida (MIC, 5 mg/mL) displayed the best inhibitory effect. However, the adherence between UTI-U7 and HeLa cells was unaffected by the ME of the E. subrigida extract. Competing Interests: Conflict of interest: All authors declare no conflicts of interest in this paper. (© 2024 the Author(s), licensee AIMS Press.)
Databáze:	MEDLINE
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