A WRI1-dependent module is essential for the accumulation of auxin and lipid in somatic embryogenesis of Arabidopsis thaliana.

Autor: Zhang WJ; National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China., Tang LP; National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China., Peng J; National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China., Zhai LM; National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China., Ma QL; National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China., Zhang XS; National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China., Su YH; National Key Laboratory of Wheat Improvement, College of Life Science, Shandong Agricultural University, Tai'an, Shandong, 271018, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 May; Vol. 242 (3), pp. 1098-1112. Date of Electronic Publication: 2024 Mar 21.
DOI: 10.1111/nph.19689
Abstrakt: The potential for totipotency exists in all plant cells; however, the underlying mechanisms remain largely unknown. Earlier findings have revealed that the overexpression of LEAFY COTYLEDON 2 (LEC2) can directly trigger the formation of somatic embryos on the cotyledons of Arabidopsis. Furthermore, cotyledon cells that overexpress LEC2 accumulate significant lipid reserves typically found in seeds. The precise mechanisms and functions governing lipid accumulation in this process remain unexplored. In this study, we demonstrate that WRINKLED1 (WRI1), the key regulator of lipid biosynthesis, is essential for somatic embryo formation, suggesting that WRI1-mediated lipid biosynthesis plays a crucial role in the transition from vegetative to embryonic development. Our findings indicate a direct interaction between WRI1 and LEC2, which enhances the enrichment of LEC2 at downstream target genes and stimulates their induction. Besides, our data suggest that WRI1 forms a complex with LEC1, LEC2, and FUSCA3 (FUS3) to facilitate the accumulation of auxin and lipid for the somatic embryo induction, through strengthening the activation of YUCCA4 (YUC4) and OLEOSIN3 (OLE3) genes. Our results uncover a regulatory module controlled by WRI1, crucial for somatic embryogenesis. These findings provide valuable insights into our understanding of plant cell totipotency.
(© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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