The impact of ZIKV infection on gene expression in neural cells over time.

Autor: Rodrigues MMS; Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Júnior AMP; Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Fukutani ER; Laboratório de Pesquisa Clínica e Translacional (LPCT), Instituto Gonçalo Moniz, Salvador, Brazil.; Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative, Salvador, Brazil., Bergamaschi KB; Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Araújo-Pereira M; Laboratório de Pesquisa Clínica e Translacional (LPCT), Instituto Gonçalo Moniz, Salvador, Brazil.; Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative, Salvador, Brazil., Salgado VR; União Metropolitana de Educação e Cultura (UNIME), Lauro de Freitas, Bahia, Brazil., de Queiroz ATL; Laboratório de Pesquisa Clínica e Translacional (LPCT), Instituto Gonçalo Moniz, Salvador, Brazil.; Multinational Organization Network Sponsoring Translational and Epidemiological Research (MONSTER) Initiative, Salvador, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Mar 21; Vol. 19 (3), pp. e0290209. Date of Electronic Publication: 2024 Mar 21 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0290209
Abstrakt: Zika virus (ZIKV) outbreak caused one of the most significant medical emergencies in the Americas due to associated microcephaly in newborns. To evaluate the impact of ZIKV infection on neuronal cells over time, we retrieved gene expression data from several ZIKV-infected samples obtained at different time point post-infection (pi). Differential gene expression analysis was applied at each time point, with more differentially expressed genes (DEG) identified at 72h pi. There were 5 DEGs (PLA2G2F, TMEM71, PKD1L2, UBD, and TNFAIP3 genes) across all timepoints, which clearly distinguished between infected and healthy samples. The highest expression levels of all five genes were identified at 72h pi. Taken together, our results indicate that ZIKV infection greatly impacts human neural cells at early times of infection, with peak perturbation observed at 72h pi. Our analysis revealed that all five DEGs, in samples of ZIKV-infected human neural stem cells, remained highly upregulated across the timepoints evaluated. Moreover, despite the pronounced inflammatory host response observed throughout infection, the impact of ZIKV is variable over time. Finally, the five DEGs identified herein play prominent roles in infection, and could serve to guide future investigations into virus-host interaction, as well as constitute targets for therapeutic drug development.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Rodrigues et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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