Mitochondria-ER contact sites expand during mitosis.

Autor:	Yu F; Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Courjaret R; Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA., Assaf L; Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.; College of Health and Life Science, Hamad bin Khalifa University, Doha, Qatar., Elmi A; Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.; College of Health and Life Science, Hamad bin Khalifa University, Doha, Qatar., Hammad A; Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.; College of Health and Life Science, Hamad bin Khalifa University, Doha, Qatar., Fisher M; Department of Cell Biology, UConn Health, 263 Farmington Ave, Farmington, CT 06030, USA., Terasaki M; Department of Cell Biology, UConn Health, 263 Farmington Ave, Farmington, CT 06030, USA., Machaca K; Calcium Signaling Group, Research Department, Weill Cornell Medicine Qatar, Education City, Qatar Foundation, Doha, Qatar.; Department of Physiology and Biophysics, Weill Cornell Medicine, New York, NY, USA.
Jazyk:	angličtina
Zdroj:	IScience [iScience] 2024 Mar 01; Vol. 27 (4), pp. 109379. Date of Electronic Publication: 2024 Mar 01 (Print Publication: 2024).
DOI:	10.1016/j.isci.2024.109379
Abstrakt:	Mitochondria-ER contact sites (MERCS) are involved in energy homeostasis, redox and Ca 2+ signaling, and inflammation. MERCS are heavily studied; however, little is known about their regulation during mitosis. Here, we show that MERCS expand during mitosis in three cell types using various approaches, including transmission electron microscopy, serial EM coupled to 3D reconstruction, and a split GFP MERCS marker. We further show enhanced Ca 2+ transfer between the ER and mitochondria using either direct Ca 2+ measurements or by quantifying the activity of Ca 2+ -dependent mitochondrial dehydrogenases. Collectively, our results support a lengthening of MERCS in mitosis that is associated with improved Ca 2+ coupling between the two organelles. This augmented Ca 2+ coupling could be important to support the increased energy needs of the cell during mitosis. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© 2024 The Authors.)
Databáze:	MEDLINE
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