Longitudinal intravital imaging of mouse placenta.

Autor: Zhu X; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Huang Q; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.; Department of Pediatric Surgery, Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710004, China.; Terasaki Institute for Biomedical Innovation, Los Angeles, CA 90024, USA., Jiang L; Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.; Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA., Nguyen VT; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Vu T; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Devlin G; Department of Surgery, Duke University School of Medicine, Durham, NC 27708, USA., Shaima J; Division of Hematologic Malignancies and Cellular Therapy, Department of Medicine, Duke University School of Medicine, Durham, NC 27708, USA., Wang X; Division of Hematologic Malignancies and Cellular Therapy, Department of Medicine, Duke University School of Medicine, Durham, NC 27708, USA., Chen Y; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Ma L; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Xiang K; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Wang E; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Rong Q; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA., Zhou Q; Department of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA.; Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA., Kang Y; Division of Hematologic Malignancies and Cellular Therapy, Department of Medicine, Duke University School of Medicine, Durham, NC 27708, USA., Asokan A; Department of Surgery, Duke University School of Medicine, Durham, NC 27708, USA., Feng L; Department of Obstetrics and Gynecology, Duke University School of Medicine, Durham, NC 27708, USA., Hsu SD; Department of Medicine, Duke University School of Medicine, Durham, NC 27708, USA., Shen X; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.; Terasaki Institute for Biomedical Innovation, Los Angeles, CA 90024, USA., Yao J; Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2024 Mar 22; Vol. 10 (12), pp. eadk1278. Date of Electronic Publication: 2024 Mar 20.
DOI: 10.1126/sciadv.adk1278
Abstrakt: Studying placental functions is crucial for understanding pregnancy complications. However, imaging placenta is challenging due to its depth, volume, and motion distortions. In this study, we have developed an implantable placenta window in mice that enables high-resolution photoacoustic and fluorescence imaging of placental development throughout the pregnancy. The placenta window exhibits excellent transparency for light and sound. By combining the placenta window with ultrafast functional photoacoustic microscopy, we were able to investigate the placental development during the entire mouse pregnancy, providing unprecedented spatiotemporal details. Consequently, we examined the acute responses of the placenta to alcohol consumption and cardiac arrest, as well as chronic abnormalities in an inflammation model. We have also observed viral gene delivery at the single-cell level and chemical diffusion through the placenta by using fluorescence imaging. Our results demonstrate that intravital imaging through the placenta window can be a powerful tool for studying placenta functions and understanding the placental origins of adverse pregnancy outcomes.
Databáze: MEDLINE