Immunochromatographic POC-CCA Test for the diagnosis of intestinal schistosomiasis in a high endemic region in Brazil: Differences in the interpretation of results.

Autor: Carvalho RG; Laboratory of Parasitology and Mollusc Biology, Department of Clinical Analysis and Toxicology, Federal University of Ceará, Rua Pastor Samuel Munguba 1210, Fortaleza, Ceará 60.430-272, Brazil., Pinheiro MCC; Laboratory of Parasitology and Mollusc Biology, Department of Clinical Analysis and Toxicology, Federal University of Ceará, Rua Pastor Samuel Munguba 1210, Fortaleza, Ceará 60.430-272, Brazil., Damasceno Morais FJ; Laboratory of Parasitology and Mollusc Biology, Department of Clinical Analysis and Toxicology, Federal University of Ceará, Rua Pastor Samuel Munguba 1210, Fortaleza, Ceará 60.430-272, Brazil; Pathology Postgraduate Program, Department of Pathology and Legal Medicine, School of Medicine, Universidade Federal do Ceará, Rua Monsenhor Furtado s/n, Fortaleza, Ceará, 60.441-750, Brazil., Galvão RLF; Laboratory of Parasitology and Mollusc Biology, Department of Clinical Analysis and Toxicology, Federal University of Ceará, Rua Pastor Samuel Munguba 1210, Fortaleza, Ceará 60.430-272, Brazil; Pathology Postgraduate Program, Department of Pathology and Legal Medicine, School of Medicine, Universidade Federal do Ceará, Rua Monsenhor Furtado s/n, Fortaleza, Ceará, 60.441-750, Brazil., Barbosa L; Parasitology and Tropical Entomology Laboratory, Department of Morphology, Federal University of Sergipe, Avenida Marechal Rondon Jardim s/n, São Cristóvão, Sergipe, 49.100-000, Brazil., Souza Sá SLC; Epidemiological Monitoring. State's Office for Health of Sergipe. Avenida Augusto Franco 3150, Aracaju, Sergipe 49097-670, Brazil., Scherr TF; Department of Chemistry, Vanderbilt University, 1234 Stevenson Center Lane, Nashville, Tennessee 37240, USA., Bezerra FSM; Laboratory of Parasitology and Mollusc Biology, Department of Clinical Analysis and Toxicology, Federal University of Ceará, Rua Pastor Samuel Munguba 1210, Fortaleza, Ceará 60.430-272, Brazil; Pathology Postgraduate Program, Department of Pathology and Legal Medicine, School of Medicine, Universidade Federal do Ceará, Rua Monsenhor Furtado s/n, Fortaleza, Ceará, 60.441-750, Brazil; Medical Sciences Postgraduate Program, Department of Internal Medicine, School of Medicine, Universidade Federal do Ceará, Rua Monsenhor Furtado s/n Fortaleza, Ceará, 60.441-75, Brazil. Electronic address: bezerra@ufc.br.
Jazyk: angličtina
Zdroj: Acta tropica [Acta Trop] 2024 Jun; Vol. 254, pp. 107181. Date of Electronic Publication: 2024 Mar 27.
DOI: 10.1016/j.actatropica.2024.107181
Abstrakt: The POC-CCA test is subject to variations in reading interpretations depending on the intensity of its results, and trace test reading have implications for determining prevalence. The aim of this study was to assess whether the readings obtained from the POC-CCA tests, conducted using a semi-quantitative scale (the G-score classification for test determination), exhibited concurrence with the direct visual interpretation (positive, negative, or trace) performed by two distinct analysts, using photographs from previously performed POC-CCA test carried out in the municipality of Maruim, in the state of Sergipe-Brazil, a region of high endemicity. The devices used to read the photographs were smartphones, so as to simulate field usage, and a desktop, a tool with higher image quality that would help the researchers in the evaluation and establishment of the final result at a later. In direct visual interpretation of the POC-CCA photographs, the most discordant results occurred in the identification of the trace response (T). The Kappa index established for the direct visual interpretation between the two analysts, in which T is considered as positive, in the desktop was κ=0.826 and in the smartphone, κ=0.950. When we use the G-score as a reading standardization technique and classify the results according to the manufacturer, with trace being evaluated as positive, the highest level of agreement was obtained. Some disagreement remains between the direct visual interpretation and the G-score when performed on the desktop, with more individuals being classified as negative in the direct visual interpretation, by both analysts. However, this result was not statistically significant. The use of the G-score scale proved to be an excellent tool for standardizing the readings and classifying the results according to the semi-quantitative scale showed greater concordance of results both among analysts and among the different devices used to view the photographs.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interest or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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