High-risk clones of carbapenem resistant Klebsiella pneumoniae recovered from pediatric patients in Southern Brazil.

Autor: Krul D; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil., Rodrigues LS; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil., Siqueira AC; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil., Mesa D; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil., Dos Santos ÉM; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil.; Hospital Pequeno Príncipe (HPP), Curitiba, Paraná, Brazil., Vasconcelos TM; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil., Spalanzani RN; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil., Cardoso R; 4Biomics.Com, Curitiba, Paraná, Brazil., Ricieri MC; Hospital Pequeno Príncipe (HPP), Curitiba, Paraná, Brazil., de Araújo Motta F; Hospital Pequeno Príncipe (HPP), Curitiba, Paraná, Brazil., Conte D; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil., Dalla-Costa LM; Faculdades Pequeno Príncipe (FPP), Curitiba, Paraná, Brazil. lmdallacosta@gmail.com.; Instituto de Pesquisa Pelé Pequeno Príncipe (IPPPP), Av. Silva Jardim, 1632 -Rebouças, Curitiba, Paraná, CEP 80250-060, Brazil. lmdallacosta@gmail.com.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2024 Jun; Vol. 55 (2), pp. 1437-1443. Date of Electronic Publication: 2024 Mar 19.
DOI: 10.1007/s42770-024-01299-w
Abstrakt: Carbapenem-resistant Klebsiella pneumoniae (CRKP) exhibit high mortality rates in pediatric patients and usually belong to international high-risk clones. This study aimed to investigate the molecular epidemiology and carbapenem resistance mechanisms of K. pneumoniae isolates recovered from pediatric patients, and correlate them with phenotypical data. Twenty-five CRKP isolates were identified, and antimicrobial susceptibility was assessed using broth microdilution. Carbapenemase production and β-lactamase genes were detected by phenotypic and genotypic tests. Multilocus sequence typing was performed to differentiate the strains and whole-genome sequencing was assessed to characterize a new sequence type. Admission to the intensive care unit and the use of catheters were significantly positive correlates of CRKP infection, and the mortality rate was 36%. Almost all isolates showed multidrug-resistant phenotype, and most frequent resistant gene was bla KPC . We observed the dissemination of ST307 and clones belonging to CG258, which are considered high risk. In pediatric patients, these clones present with high genomic plasticity, favoring adaptation of the KPC and NDM enzymes to healthcare environments.
(© 2024. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.)
Databáze: MEDLINE
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