Structural insights into vesicular monoamine storage and drug interactions.

Autor: Ye J; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO, USA., Chen H; Department of Radiology, Washington University School of Medicine, St Louis, MO, USA., Wang K; Department of Biomedical Sciences, University of Copenhagen, Copenhagen, Denmark., Wang Y; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO, USA., Ammerman A; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO, USA., Awasthi S; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO, USA., Xu J; Department of Radiology, Washington University School of Medicine, St Louis, MO, USA., Liu B; The Hormel Institute, University of Minnesota, Austin, MN, USA. liu00794@umn.edu., Li W; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St Louis, MO, USA. weikai@wustl.edu.
Jazyk: angličtina
Zdroj: Nature [Nature] 2024 May; Vol. 629 (8010), pp. 235-243. Date of Electronic Publication: 2024 Mar 18.
DOI: 10.1038/s41586-024-07290-7
Abstrakt: Biogenic monoamines-vital transmitters orchestrating neurological, endocrinal and immunological functions 1-5 -are stored in secretory vesicles by vesicular monoamine transporters (VMATs) for controlled quantal release 6,7 . Harnessing proton antiport, VMATs enrich monoamines around 10,000-fold and sequester neurotoxicants to protect neurons 8-10 . VMATs are targeted by an arsenal of therapeutic drugs and imaging agents to treat and monitor neurodegenerative disorders, hypertension and drug addiction 1,8,11-16 . However, the structural mechanisms underlying these actions remain unclear. Here we report eight cryo-electron microscopy structures of human VMAT1 in unbound form and in complex with four monoamines (dopamine, noradrenaline, serotonin and histamine), the Parkinsonism-inducing MPP + , the psychostimulant amphetamine and the antihypertensive drug reserpine. Reserpine binding captures a cytoplasmic-open conformation, whereas the other structures show a lumenal-open conformation stabilized by extensive gating interactions. The favoured transition to this lumenal-open state contributes to monoamine accumulation, while protonation facilitates the cytoplasmic-open transition and concurrently prevents monoamine binding to avoid unintended depletion. Monoamines and neurotoxicants share a binding pocket that possesses polar sites for specificity and a wrist-and-fist shape for versatility. Variations in this pocket explain substrate preferences across the SLC18 family. Overall, these structural insights and supporting functional studies elucidate the mechanism of vesicular monoamine transport and provide the basis to develop therapeutics for neurodegenerative diseases and substance abuse.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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