| Autor: |
Prylutska S; Department of Plants Physiology, Biochemistry and Bioenergetics, National University of Life and Environmental Sciences of Ukraine, 03041 Kyiv, Ukraine., Grebinyk A; Deutsches Elektronen-Synchrotron DESY, Platanenallee 6, 15738 Zeuthen, Germany.; Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany., Ponomarenko S; Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine., Gövem D; Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany., Chumachenko V; Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine., Kutsevol N; Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine., Petrovsky M; Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine., Ritter U; Institute of Chemistry and Biotechnology, Technical University of Ilmenau, 98693 Ilmenau, Germany., Frohme M; Division Molecular Biotechnology and Functional Genomics, Technical University of Applied Sciences Wildau, 15745 Wildau, Germany., Piosik J; Intercollegiate Faculty of Biotechnology, University of Gdańsk and Medical University of Gdańsk, 80-307 Gdańsk, Poland., Prylutskyy Y; Department of Biophysics and Neurobiology, Department of Chemistry, Taras Shevchenko National University of Kyiv, 01601 Kyiv, Ukraine. |
| Abstrakt: |
The application of a biocompatible polymer nanocarrier can provide target delivery to tumor tissues, improved pharmacokinetics, controlled drug release, etc. Therefore, the proposed strategy was to use the water-soluble star-like copolymers with a Dextran core and Poly(N-isopropylacrylamide) grafts (D-g-PNIPAM) for conjugation with the widely used chemotherapy drugs in oncology-Cisplatin (Cis-Pt) and Doxorubicin (Dox). The molecular characteristics of the copolymer were received using size-exclusion chromatography. The physicochemical characterization of the D-g-PNIPAM-Cis-Pt (or Dox) nanosystem was conducted using dynamic light scattering and FTIR spectroscopy. Using traditional biochemical methods, a comparative analysis of the enhancement of the cytotoxic effect of free Cis-Pt and Dox in combination with D-g-PNIPAM copolymers was performed in cancer cells of the Lewis lung carcinoma line, which are both sensitive and resistant to Dox; in addition, the mechanism of their action in vitro was evaluated. |
