Corrected and republished from: "Extracellular Vesicle Formation in Cryptococcus deuterogattii Impacts Fungal Virulence".

Autor:	Castelli RF; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.; Programa de Pós-Graduação em Biologia Parasitária, Instituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Brazil., Pereira A; Instituto de Química, Universidade de Campinas, São Paulo, Brazil., Honorato L; Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Valdez A; Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., de Oliveira HC; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil., Bazioli JM; Instituto de Química, Universidade de Campinas, São Paulo, Brazil.; Faculty of Pharmaceutical Sciences, State University of Campinas, Campinas, São Paulo, Brazil., Garcia AWA; Programa de Pós-graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Klimeck TDF; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil., Reis FCG; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.; Centro de Desenvolvimento Tecnológico em Saúde (CDTS), Fundação Oswaldo Cruz, Rio de Janeiro, Brazil., Camillo-Andrade AC; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil., Santos MDM; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.; Analytical Biochemistry and Proteomics Unit. IIBCE/Institut Pasteur de Montevideo, Montevideo, Uruguay., Carvalho PC; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil., Zaragoza O; Mycology Reference Laboratory. National Centre for Microbiology. Instituto de Salud Carlos III, Madrid, Spain.; Center for Biomedical Research in Network in Infectious Diseases, CB21/13/00105, Instituto de Salud Carlos III, Madrid, Spain., Staats CC; Programa de Pós-graduação em Biologia Celular e Molecular, Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil., Nimrichter L; Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil., Fill TP; Instituto de Química, Universidade de Campinas, São Paulo, Brazil., Rodrigues ML; Instituto Carlos Chagas, Fundação Oswaldo Cruz (Fiocruz), Curitiba, Brazil.; Instituto de Microbiologia Paulo de Góes (IMPG), Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2024 Apr 09; Vol. 92 (4), pp. e0003724. Date of Electronic Publication: 2024 Mar 12.
DOI:	10.1128/iai.00037-24
Abstrakt:	Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles are only superficially known. NOP16 is a eukaryotic gene that is required for the activity of benzimidazoles against Cryptococcus deuterogattii . In this study, during the phenotypic characterization of C. deuterogattii mutants expected to lack NOP16 expression, we observed a reduced EV production. Whole-genome sequencing, RNA-Seq, and cellular proteomics revealed that, contrary to our initial findings, these mutants expressed Nop16 but exhibited altered expression of 14 genes potentially involved in sugar transport. Based on this observation, we designated these mutant strains as Past1 and Past2, representing p otentially a ltered s ugar t ransport. Analysis of the small molecule composition of EVs produced by wild-type cells and the Past1 and Past2 mutant strains revealed not only a reduced number of EVs but also an altered small molecule composition. In a Galleria mellonella model of infection, the Past1 and Past2 mutant strains were hypovirulent. The hypovirulent phenotype was reverted when EVs produced by wild-type cells, but not mutant EVs, were co-injected with the mutant cells in G. mellonella . These results connect EV biogenesis, cargo, and cryptococcal virulence. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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