Suspension cell cultures of Panax vietnamensis as a biotechnological source of ginsenosides: growth, cytology, and ginsenoside profile assessment.

Autor:	Titova MV; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Lunkova MK; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Tyurina TM; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Prudnikova ON; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Popova EV; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Klychnikov OI; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia.; Department of Biochemistry, Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia., Metalnikov PS; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia., Ikhalaynen YA; Department of Analytical Chemistry, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia., Vasileva EN; Department of Analytical Chemistry, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia., Rodin IA; Department of Analytical Chemistry, Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia., Nosov AM; K.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia.; Department of Plant Physiology, Faculty of Biology, M.V. Lomonosov Moscow State University, Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 Feb 26; Vol. 15, pp. 1349494. Date of Electronic Publication: 2024 Feb 26 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1349494
Abstrakt:	Introduction: Panax vietnamensis is a valuable medicinal plant and a source of a broad spectrum of biologically active ginsenosides of different structural groups. Overexploitation and low adaptability to planation cultivation have made this species vulnerable to human pressure and prompted the development of cell cultivation in vitro as a sustainable alternative to harvesting wild plants for their bioactive components. Despite high interest in biotechnological production, little is known about the main factors affecting cell growth and ginsenoside biosynthesis of this species under in vitro conditions. In this study, the potential of cell cultures of P. vietnamensis as a biotechnological source of ginsenosides was was assessed. Methods: Six suspension cell lines that were developed from different sections of a single rhizome through a multi-step culture optimization process and maintained for over 3 years on media with different mineral salt base and varying contents of auxins and cytokinins. These cell lines were evaluated for productivity parameters and cytological characteristics. Ginsenoside profiles were assessed using a combination of the reversed-phase ultra-high-performance liquid chromatography-Orbitrap-tandem mass spectrometry (UHPLC-Orbitrap-MS/MS) and ultra-performance liquid chromatography-time of flight-mass spectrometry (UPLC-TOF-MS). Results: All lines demonstrated good growth with a specific growth rate of 0.1-0.2 day -1 , economic coefficient of 0.31-0.70, productivity on dry weight (DW) of 0.30-0.83 gDW (L·day) -1 , and maximum biomass accumulation varying from 10 to 22 gDW L -1 . Ginsenosides of the protopanaxadiol (Rb1, Rb2/Rb3, malonyl-Rb1, and malonyl-Rb2/Rb3), oleanolic acid (R0 and chikusetsusaponin IV), and ocotillol (vinaginsenoside R1) groups and their isomers were identified in cell biomass extracts. Chikusetsusaponin IV was identified in P. vietnamensis cell culture for the first time. Discussion: These results suggest that suspension cell cultures of Vietnamese ginseng have a high potential for the biotechnological production of biomass containing ginsenosides, particularly of the oleanolic acid and ocotillol groups. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Titova, Lunkova, Tyurina, Prudnikova, Popova, Klychnikov, Metalnikov, Ikhalaynen, Vasileva, Rodin and Nosov.)
Databáze:	MEDLINE
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