Ensemble Detection of DNA Engineering Signatures.

Autor:	Adler A; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Bader JS; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States., Basnight B; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Booth BW; Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States., Cai J; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States., Cho E; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States., Collins JH; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, United States., Ge Y; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States., Grothendieck J; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Keating K; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, United States., Marshall T; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Persikov A; Department of Computer Science, Princeton University, Princeton, New Jersey 08544, United States., Scott H; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Siegelmann R; Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21218, United States., Singh M; Department of Computer Science, Princeton University, Princeton, New Jersey 08544, United States., Taggart A; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Toll B; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Wan KH; Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States., Wyschogrod D; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Yaman F; Raytheon BBN, Cambridge, Massachusetts 02138, United States., Young EM; Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, Massachusetts 01609, United States., Celniker SE; Lawrence Berkeley National Laboratory, Berkeley, California 94720, United States., Roehner N; Raytheon BBN, Cambridge, Massachusetts 02138, United States.
Jazyk:	angličtina
Zdroj:	ACS synthetic biology [ACS Synth Biol] 2024 Apr 19; Vol. 13 (4), pp. 1105-1115. Date of Electronic Publication: 2024 Mar 12.
DOI:	10.1021/acssynbio.3c00398
Abstrakt:	Synthetic biology is creating genetically engineered organisms at an increasing rate for many potentially valuable applications, but this potential comes with the risk of misuse or accidental release. To begin to address this issue, we have developed a system called GUARDIAN that can automatically detect signatures of engineering in DNA sequencing data, and we have conducted a blinded test of this system using a curated Test and Evaluation (T&E) data set. GUARDIAN uses an ensemble approach based on the guiding principle that no single approach is likely to be able to detect engineering with perfect accuracy. Critically, ensembling enables GUARDIAN to detect sequence inserts in 13 target organisms with a high degree of specificity that requires no subject matter expert (SME) review.
Databáze:	MEDLINE
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