Universal recording of immune cell interactions in vivo.

Autor: Nakandakari-Higa S; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA., Walker S; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA.; Department of Quantitative and Computational Biology, Princeton University, Princeton, NJ, USA., Canesso MCC; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA.; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., van der Heide V; Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Tisch Cancer Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Chudnovskiy A; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA., Kim DY; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., Jacobsen JT; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA.; Institute for Immunology and Transfusion Medicine, Rikshospitalet, University of Oslo, Oslo, Norway., Parsa R; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., Bilanovic J; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA., Parigi SM; Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY, USA., Fiedorczuk K; Laboratory of Membrane Biology and Biophysics, The Rockefeller University, New York, NY, USA., Fuchs E; Laboratory of Mammalian Cell Biology and Development, The Rockefeller University, New York, NY, USA.; Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA., Bilate AM; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA., Pasqual G; Laboratory of Synthetic Immunology, Department of Surgery, Oncology and Gastroenterology, University of Padova, Padua, Italy., Mucida D; Laboratory of Mucosal Immunology, The Rockefeller University, New York, NY, USA.; Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA., Kamphorst AO; Marc and Jennifer Lipschultz Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Tisch Cancer Center, Icahn School of Medicine at Mount Sinai, New York, NY, USA.; Department of Oncological Sciences, Icahn School of Medicine at Mount Sinai, New York, NY, USA., Pritykin Y; Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ, USA. pritykin@princeton.edu.; Department of Computer Science, Princeton University, Princeton, NJ, USA. pritykin@princeton.edu., Victora GD; Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA. victora@rockefeller.edu.
Jazyk: angličtina
Zdroj: Nature [Nature] 2024 Mar; Vol. 627 (8003), pp. 399-406. Date of Electronic Publication: 2024 Mar 06.
DOI: 10.1038/s41586-024-07134-4
Abstrakt: Immune cells rely on transient physical interactions with other immune and non-immune populations to regulate their function 1 . To study these 'kiss-and-run' interactions directly in vivo, we previously developed LIPSTIC (labelling immune partnerships by SorTagging intercellular contacts) 2 , an approach that uses enzymatic transfer of a labelled substrate between the molecular partners CD40L and CD40 to label interacting cells. Reliance on this pathway limited the use of LIPSTIC to measuring interactions between CD4 + T helper cells and antigen-presenting cells, however. Here we report the development of a universal version of LIPSTIC (uLIPSTIC), which can record physical interactions both among immune cells and between immune and non-immune populations irrespective of the receptors and ligands involved. We show that uLIPSTIC can be used, among other things, to monitor the priming of CD8 + T cells by dendritic cells, reveal the steady-state cellular partners of regulatory T cells and identify germinal centre-resident T follicular helper cells on the basis of their ability to interact cognately with germinal centre B cells. By coupling uLIPSTIC with single-cell transcriptomics, we build a catalogue of the immune populations that physically interact with intestinal epithelial cells at the steady state and profile the evolution of the interactome of lymphocytic choriomeningitis virus-specific CD8 + T cells in multiple organs following systemic infection. Thus, uLIPSTIC provides a broadly useful technology for measuring and understanding cell-cell interactions across multiple biological systems.
(© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)
Databáze: MEDLINE
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