Structural and in vivo-in vitro myocardial injury protection features of two novel polysaccharides from Allium macrostemon Bunge and Allium chinense G. Don.

Autor: Qin Z; Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China., Huang M; Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China., Zhang X; Departments of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China., Hua Y; College of Medicine, Henan Engineering Research Center of Funiu Mountain's Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China., Zhang X; Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Engineering Research Center for Application & Translation of Precision Clinical Pharmacy, Zhengzhou 450052, China., Li X; Department of Pathology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China., Fan C; College of Medicine, Henan Engineering Research Center of Funiu Mountain's Medicinal Resources Utilization and Molecular Medicine, Pingdingshan University, Pingdingshan 467000, China., Li R; Departments of Hepatobiliary and Pancreatic Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China. Electronic address: wangzihengboy@163.com., Yang J; Department of Pharmacy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Henan Engineering Research Center for Application & Translation of Precision Clinical Pharmacy, Zhengzhou 450052, China. Electronic address: jingyang_0101@163.com.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Apr; Vol. 264 (Pt 1), pp. 130537. Date of Electronic Publication: 2024 Mar 02.
DOI: 10.1016/j.ijbiomac.2024.130537
Abstrakt: This study aimed to investigate the structural characteristics, in vivo antiatherosclerosis activity, and in vitro myocardial injury protection effects of polysaccharides from Allium macrostemon Bunge and Allium chinense G. Don. Thus, crude polysaccharides of Allium macrostemon Bunge and Allium chinense G. Don significantly reduced serum lipid levels, improved cardiac myocyte morphology and arrangement, and relieved the development of myocardial fibrosis. Meanwhile, the lesion areas of the aorta and aortic valve had evident visual improvements. Furthermore, two main novel purified polysaccharides, namely, AMB-1 and ACGD-1, were isolated and characterized from crude Allium macrostemon Bunge and Allium chinense G. Don fractions, respectively. The purified polysaccharides mainly consisted of fructose and glucose and had molecular weights of 25.22 and 19.53 kDa, respectively. In addition, Fourier transform infrared spectroscopy, methylation, and nuclear magnetic resonance data revealed the primary structures of the AMB1 (or ACGD1) backbone with branched side chains. Scanning electron microscope analysis showed that the purified polysaccharides were both piled together in a lamellar or clastic form with a smooth surface along with linear or irregular bulges. Moreover, the purified polysaccharides both showed nontoxicity on H9c2 cells and effectively dropped hypoxia/reoxygenation-induced apoptosis by the BCL-2/BAX pathway. Overall, the characterization of the structural properties and in vivo and in vitro myocardial injury protection effects of Allium macrostemon Bunge and Allium chinense G. Don polysaccharides enriched our understanding of their nutritional and medicinal values. To the best of our knowledge, this is the first study on the structural characteristics and bioactivities of Allium chinense G. Don polysaccharides.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest. The experiment was performed and approved in accordance with the guidelines of Laboratory Animal Ethics Committee of Zhengzhou University, which comply with national and international scientific and ethical guidelines.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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