Use of PCR-DGGE-Based Molecular Methods to Analyze Nematode Community Diversity.
| Autor: | Festa M; CNR - Istituto di Biofisica, Genova, Italy., Abbruscato P; Nuova Genetica Italiana, Villa Guardia, CO, Italy., Manachini B; Department of Agricultural, Food and Forest Sciences, University of Palermo, Palermo, Italy. barbara.manachini@unipa.it. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2024; Vol. 2756, pp. 247-255. |
| DOI: | 10.1007/978-1-0716-3638-1_6 |
| Abstrakt: | DGGE (denaturing gradient gel electrophoresis) is a nucleic acid separation technique applied to the evaluation of microbial biodiversity. This technique is quite rapid and cheap compared to other types of analysis. Here we describe the comparison of nematode communities inhabiting different ecosystems. After an ecologically representative sampling collection and the nematode extraction from soil, nematodes are centrifuged in Eppendorf tubes to facilitate DNA extraction. DNA from the whole community of each type of soil is extracted, amplified with primers for 18 S rDNA and used in DGGE analysis. The profiles of DGGE can be analyzed with appropriate software, and biodiversity indices can be estimated. (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| Databáze: | MEDLINE |
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