Molecular identification of Haemonchus contortus in sheep from Upper Egypt.
| Autor: | Mohamed SA; Department of Parasitology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt., Dyab AK; Department of Parasitology, Faculty of Medicine, Assiut University, Assiut, Egypt., Raya-Álvarez E; Rheumatology Department, Hospital Universitario San Cecilio, Av. de la Investigación, Granada, Spain., Abdel-Aziz FM; Department of Parasitology, Faculty of Veterinary Medicine, Assiut University, Assiut, Egypt., Osman F; Department of Parasitology, Animal Health Research Institute, Agriculture Research Center (ARC), Giza, Egypt., Gareh A; Department of Parasitology, Faculty of Veterinary Medicine, Aswan University, Aswan, Egypt., Farag AMM; Department of Internal Medicine and Infectious Diseases Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt., Salman D; Department of Animal Medicine, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt., El-Khadragy MF; Department of Biology, College of Science, Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia., Bravo-Barriga D; Departamento de Sanidad Animal, Grupo de Investigación en Salud Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes (ENZOEM), Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain., Agil A; Department of Pharmacology, Biohealth Institute Granada (IBs Granada) and Neuroscience Institute, School of Medicine, University of Granada, Granada, Spain., Elmahallawy EK; Departamento de Sanidad Animal, Grupo de Investigación en Salud Animal y Zoonosis (GISAZ), UIC Zoonosis y Enfermedades Emergentes (ENZOEM), Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain.; Department of Zoonoses, Faculty of Veterinary Medicine, Sohag University, Sohag, Egypt. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in veterinary science [Front Vet Sci] 2024 Feb 12; Vol. 10, pp. 1327424. Date of Electronic Publication: 2024 Feb 12 (Print Publication: 2023). |
| DOI: | 10.3389/fvets.2023.1327424 |
| Abstrakt: | Introduction: Haemonchus spp. are considered the most important strongylid nematodes with a worldwide distribution. The parasite's blood-sucking nature can lead to severe anemia in infected animals. Despite its widespread impact, there is a dearth of comprehensive data on morphological and molecular identification methods for Haemonchus spp. in sheep from Upper Egypt. To address this gap, our current study aimed to assess the prevalence of Haemonchus spp. in 400 sheep fecal samples. Methods: We employed microscopic examination and molecular techniques, using polymerase chain reaction (PCR) targeting the 18S gene for precise identification. Additionally, the potential risk factors associated with the infection by the parasite in sheep were explored. Results: The study pointed out that 33.00% (132 of 400) of the examined sheep were infected with Haemonchus spp. Sheep age and seasonal variability were found to be significant factors ( p < 0.05) associated with the infection. Notably, sheep under 2 years old exhibited a higher risk, with an infection rate of 43.75% (84 out of 192), than their older counterparts. Furthermore, all reported infections were exclusively observed during the cold season, constituting 58.93% (132 out of 224) of cases. By contrast, no statistically significant association ( p > 0.05) was found between the sex of the examined sheep and the occurrence of haemonchosis. Employing molecular methods, we isolated and identified the parasite through PCR analysis of cultured larvae, which were then subsequently confirmed as Haemonchus contortus via phylogenetic analysis. Discussion: The study concluded that there was a relatively high occurrence of H. contortu s among sheep from Upper Egypt. We recommend the implementation of stringent and effective control measures to combat the infection and safeguard livestock health. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Mohamed, Dyab, Raya-Álvarez, Abdel-Aziz, Osman, Gareh, Farag, Salman, El-Khadragy, Bravo-Barriga, Agil and Elmahallawy.) |
| Databáze: | MEDLINE |
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