Most axonal mitochondria in cortical pyramidal neurons lack mitochondrial DNA and consume ATP.

Autor:	Hirabayashi Y; Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo; Tokyo, 113-8656, Japan., Lewis TL Jr; Aging & Metabolism Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA., Du Y; Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo; Tokyo, 113-8656, Japan., Virga DM; Department of Biological Sciences, Columbia University; New York, NY, 10027, USA.; Department of Neuroscience, Columbia University; New York, NY, 10027, USA.; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University; New York, NY, 10027, USA., Decker AM; Department of Biological Sciences, Columbia University; New York, NY, 10027, USA., Coceano G; Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden., Alvelid J; Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden.; Department of Biophysical Imaging, Leibniz Institute of Photonic Technology, Jena, Germany., Paul MA; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University; New York, NY, 10027, USA.; Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS, INSERM, Université PSL; Paris, France., Hamilton S; Department of Neuroscience, Columbia University; New York, NY, 10027, USA.; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University; New York, NY, 10027, USA., Kneis P; Aging & Metabolism Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA., Takahashi Y; Department of Electronics, Graduate School of Engineering, Nagoya University, 464-8603, Nagoya, Japan.; Nano Life Science Institute (WPI-NanoLSI), Kanazawa University, Kakuma-machi, Kanazawa, Ishikawa, 920-1192 Japan., Gaublomme JT; Department of Biological Sciences, Columbia University; New York, NY, 10027, USA., Testa I; Department of Applied Physics and SciLifeLab, KTH Royal Institute of Technology, Stockholm, Sweden., Polleux F; Department of Neuroscience, Columbia University; New York, NY, 10027, USA.; Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University; New York, NY, 10027, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 Feb 13. Date of Electronic Publication: 2024 Feb 13.
DOI:	10.1101/2024.02.12.579972
Abstrakt:	In neurons of the mammalian central nervous system (CNS), axonal mitochondria are thought to be indispensable for supplying ATP during energy-consuming processes such as neurotransmitter release. Here, we demonstrate using multiple, independent, in vitro and in vivo approaches that the majority (~80-90%) of axonal mitochondria in cortical pyramidal neurons (CPNs), lack mitochondrial DNA (mtDNA). Using dynamic, optical imaging analysis of genetically encoded sensors for mitochondrial matrix ATP and pH, we demonstrate that in axons of CPNs, but not in their dendrites, mitochondrial complex V (ATP synthase) functions in a reverse way, consuming ATP and protruding H + out of the matrix to maintain mitochondrial membrane potential. Our results demonstrate that in mammalian CPNs, axonal mitochondria do not play a major role in ATP supply, despite playing other functions critical to regulating neurotransmission such as Ca 2+ buffering. Competing Interests: Competing interests: Authors declare that they have no competing interests.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu