| Autor: |
Hassan A; Department of Bioinformatics, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat 32897, Egypt., Rijo P; CBIOS-Lusófona University's Research Center for Biosciences and Health Technologies, 1749-024 Lisbon, Portugal.; Instituto de Investigação do Medicamento (iMed.ULisboa), Faculdade de Farmácia, Universidade de Lisboa, 1649-003 Lisbon, Portugal., Abuamara TMM; Department of Basic Medical Science, Faculty of Dentistry, Al-Ahliyya Amman University, Amman 19111, Jordan.; Department of Histology, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt., Ali Lashin LS; Department of Basic Medical Science, Faculty of Dentistry, Al-Ahliyya Amman University, Amman 19111, Jordan.; Department of Medical Physiology, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt., Kamar SA; Department of Basic Medical Science, Faculty of Dentistry, Al-Ahliyya Amman University, Amman 19111, Jordan.; Department of Anatomy and Embryology, Faculty of Medicine, Ain Shams University, Cairo 11566, Egypt., Bangay G; CBIOS-Lusófona University's Research Center for Biosciences and Health Technologies, 1749-024 Lisbon, Portugal.; Universidad de Alcalá de Henares. Facultad de Farmacia, Departamento de Ciencias Biomédicas (Área de Farmacología; Nuevos agentes antitumorales, Acción tóxica sobre células leucémicas), Ctra. Madrid-Barcelona km. 33,600, 28805 Alcalá de Henares, Madrid, España., Al-Sawahli MM; Department of Pharmaceutics, College of Pharmacy, The Islamic University, Najaf 54001, Iraq.; Department of Pharmaceutical Technology, Faculty of Pharmacy, Kafr Elsheikh University, Kafr Elsheikh 33516, Egypt., Fouad MK; College of Biotechnology, Misr University of Science and Technology, Giza 12573, Egypt., Zoair MA; Department of Physiology, Faculty of Medicine, Al-Azhar University, Cairo 11884, Egypt., Abdalrhman TI; Department of Histology, Faculty of Medicine, Al-Azhar University, Assiut 71524, Egypt., Elebeedy D; College of Biotechnology, Misr University of Science and Technology, Giza 12573, Egypt., Ibrahim IA; Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat 32897, Egypt., Mohamed AF; Holding Company for Vaccine and Sera Production (VACSERA), Giza 22311, Egypt., Abd El Maksoud AI; College of Biotechnology, Misr University of Science and Technology, Giza 12573, Egypt.; Department of Industrial Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat 32897, Egypt. |
| Abstrakt: |
Nonalcoholic fatty liver disease (NAFLD) is a major hepatic disorder occurring in non-alcohol-drinking individuals. Salvianic acid A or Danshensu (DSS, 3-(3, 4-dihydroxyphenyl)-(2 R )-lactic acid), derived from the root of Danshen ( Salvia miltiorrhiza ), has demonstrated heart and liver protective properties. In this work, we investigated the antioxidant activity and hepatoprotective activity of Danshensu alone and in combination with different agents, such as probiotic bacteria ( Lactobacillus casei and Lactobacillus acidophilus ), against several assays. The inhibition mechanism of the methylation gene biomarkers, such as DNMT-1, MS, STAT-3, and TET-1, against DSS was evaluated by molecular docking and RT-PCR techniques. The physicochemical and pharmacokinetic ADMET properties of DSS were determined by SwissADME and pkCSM. The results indicated that all lipid blood test profiles, including cholesterol (TC), triglycerides (TG), low-density lipoprotein cholesterol (LDL-C), and high-density lipoprotein cholesterol (HDL-C), were reduced after the oral administration of Danshensu combined with probiotics ( L. casei and L. acidophilus ) that demonstrated good, efficient free radical scavenging activity, measured using anti-oxidant assays. ADMET and drug-likeness properties certify that the DSS could be utilized as a feasible drug since DSS showed satisfactory physicochemical and pharmacokinetic ADMET properties. |
