Increased levels of advanced oxidation protein products (AOPPs) were associated with nociceptive behavior and clinical scores in an experimental progressive autoimmune encephalomyelitis model (PMS-EAE).

Autor: Rodrigues P; Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Frare JM; Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Peres DS; Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Viero FT; Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Ruviaro NA; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Dos Santos Stein C; Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., da Silva Brum E; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Moresco RN; Graduate Program in Pharmaceutical Sciences, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Oliveira SM; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Bochi GV; Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil., Trevisan G; Graduate Program in Pharmacology, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.; Graduate Program in Biological Sciences: Toxicological Biochemistry, Federal University of Santa Maria (UFSM), Santa Maria, RS, Brazil.
Jazyk: angličtina
Zdroj: Journal of neurochemistry [J Neurochem] 2024 Jun; Vol. 168 (6), pp. 1143-1156. Date of Electronic Publication: 2024 Feb 19.
DOI: 10.1111/jnc.16081
Abstrakt: Multiple sclerosis (MS) is a neurodegenerative disease that affects the central nervous system (CNS) generating neuropathic pain and anxiety. Primary progressive MS (PPMS) is the most disabling clinical form, and the patients present an intense neurodegenerative process. In this context, the advanced oxidation protein products (AOPPs) are oxidized compounds and their accumulation in plasma has been related to clinical disability in MS patients. However, the involvement of AOPPs in neuropathic pain- and anxiety-like symptoms was not previously evaluated. To assess this, female mice C57BL/6J were used to induce progressive experimental autoimmune encephalomyelitis (PMS-EAE). Clinical score, weight, strength of plantar pressure, rotarod test, mechanical allodynia, and cold hypersensitivity were evaluated before induction (baseline) and on days 7 th , 10 th , and 14 th post-immunization. We assessed nest building, open field, and elevated plus-maze tests 13 days post-immunization. Animals were killed at 14 days post-immunization; then, AOPPs levels, NADPH oxidase, and myeloperoxidase (MPO) activity were measured in the prefrontal cortex, hippocampus, and spinal cord samples. The clinical score increased 14 th post-immunization without changes in weight and mobility. Reduced paw strength, mechanical allodynia, and cold allodynia increased in the PMS-EAE animals. PMS-EAE mice showed spontaneous nociception and anxiety-like behavior. AOPPs concentration, NADPH oxidase, and MPO activity increase in CNS structures. Multivariate analyses indicated that the rise of AOPPs levels, NADPH oxidase, and MPO activity influenced the clinical score and cold allodynia. Thus, we indicated the association between non-stimuli painful perception, anxiety-like, and CNS oxidative damage in the PMS-EAE model.
(© 2024 International Society for Neurochemistry.)
Databáze: MEDLINE
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