Electrochemical biosensor for the evaluation of monoclonal antibodies targeting the N protein of SARS-CoV-2 virus.

Autor: Liustrovaite V; NanoTechnas - Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University (VU), Naugarduko St. 24, LT-03225 Vilnius, Lithuania; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University (VU), Naugarduko St. 24, LT-03225 Vilnius, Lithuania., Drobysh M; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University (VU), Naugarduko St. 24, LT-03225 Vilnius, Lithuania; Department of Nanotechnology, State Research Institute Center for Physical and Technological Sciences (FTMC), Sauletekio Ave. 3, Vilnius, Lithuania., Ratautaite V; Department of Nanotechnology, State Research Institute Center for Physical and Technological Sciences (FTMC), Sauletekio Ave. 3, Vilnius, Lithuania., Ramanaviciene A; NanoTechnas - Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University (VU), Naugarduko St. 24, LT-03225 Vilnius, Lithuania., Rimkute A; Institute of Biotechnology, Life Sciences Center, Vilnius University (VU), Sauletekio Ave. 7, Vilnius, Lithuania., Simanavicius M; Institute of Biotechnology, Life Sciences Center, Vilnius University (VU), Sauletekio Ave. 7, Vilnius, Lithuania., Dalgediene I; Institute of Biotechnology, Life Sciences Center, Vilnius University (VU), Sauletekio Ave. 7, Vilnius, Lithuania., Kucinskaite-Kodze I; Institute of Biotechnology, Life Sciences Center, Vilnius University (VU), Sauletekio Ave. 7, Vilnius, Lithuania., Plikusiene I; NanoTechnas - Center of Nanotechnology and Materials Science, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University (VU), Naugarduko St. 24, LT-03225 Vilnius, Lithuania., Chen CF; Institute of Applied Mechanics, National Taiwan University, Taipei City 106, Taiwan. Electronic address: stevechen@iam.ntu.edu.tw., Viter R; Institute of Atomic Physics and Spectroscopy, University of Latvia, 19 Raina Blvd., Riga, LV 1586, Latvia; Center for Collective Use of Scientific Equipment, Sumy State University, 31, Sanatornaya st., 40018 Sumy, Ukraine. Electronic address: roman.viter@lu.lv., Ramanavicius A; Department of Physical Chemistry, Institute of Chemistry, Faculty of Chemistry and Geosciences, Vilnius University (VU), Naugarduko St. 24, LT-03225 Vilnius, Lithuania; Department of Nanotechnology, State Research Institute Center for Physical and Technological Sciences (FTMC), Sauletekio Ave. 3, Vilnius, Lithuania. Electronic address: arunas.ramanavicius@chf.vu.lt.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 May 10; Vol. 924, pp. 171042. Date of Electronic Publication: 2024 Feb 16.
DOI: 10.1016/j.scitotenv.2024.171042
Abstrakt: The emergence of COVID-19 caused by the coronavirus SARS-CoV-2 has prompted a global pandemic that requires continuous research and monitoring. This study presents a design of an electrochemical biosensing platform suitable for the evaluation of monoclonal antibodies targeting the SARS-CoV-2 nucleocapsid (N) protein. Screen-printed carbon electrodes (SPCE) modified with gold nanostructures (AuNS) were applied to design a versatile and sensitive sensing platform. Electrochemical techniques, including electrochemical impedance spectroscopy (EIS) and square wave voltammetry (SWV), were used to investigate the interactions between immobilised recombinant N (rN) protein and several monoclonal antibodies (mAbs). The electrochemical characterisation of SPCE/AuNS/rN demonstrated a successful immobilisation of rN, enhancing the electron transfer kinetics. Affinity interactions between immobilised rN and four mAbs (mAb-4B3, mAb-4G6, mAb-12B2, and mAb-1G5) were explored. Although mAb-4B3 showed some non-linearity, the other monoclonal antibodies exhibited specific and well-defined interactions followed by the formation of an immune complex. The biosensing platform demonstrated high sensitivity in the linear range (LR) from 0.2 nM to 1 nM with limits of detection (LOD) ranging from 0.012 nM to 0.016 nM for mAb-4G6, mAb-12B2, and mAb-1G5 and limits of quantification (LOQ) values ranging from 0.035 nM to 0.139 nM, as determined by both EIS and SWV methods. These results highlight the system's potential for precise and selective detection of monoclonal antibodies specific to the rN. This electrochemical biosensing platform provides a promising route for the sensitive and accurate detection of monoclonal antibodies specific to the rN protein.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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