Membrane vesicles in Acidithiobacillia class extreme acidophiles: influence on collective behaviors of ' Fervidacidithiobacillus caldus '.

Autor:	Rossoni S; Centro Científico y Tecnológico Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile., Beard S; Centro Científico y Tecnológico Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile.; Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile., Segura-Bidermann MI; Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile., Duarte-Ramírez J; Centro Científico y Tecnológico Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile.; Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile., Osorio FK; Instituto de Ingeniería Biológica y Médica, Escuelas de Ingeniería, Medicina y Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile., Varas-Godoy M; Centro Científico y Tecnológico Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile.; Cancer Cell Biology Lab., Centro de Biología Celular y Biomedicina (CEBICEM), Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile., Martínez-Bellange P; Biomining Advisor, Santiago, Chile., Vera M; Instituto de Ingeniería Biológica y Médica, Escuelas de Ingeniería, Medicina y Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.; Departamento de Ingeniería de Minería, Escuela de Ingeniería, Pontificia Universidad Católica de Chile, Santiago, Chile., Quatrini R; Centro Científico y Tecnológico Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile.; Facultad de Medicina y Ciencia, Universidad San Sebastián, Santiago, Chile., Castro M; Centro Científico y Tecnológico Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile.; Instituto Milenio de Oceanografía (IMO), Universidad de Concepción, Concepción, Chile.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2024 Jan 26; Vol. 14, pp. 1331363. Date of Electronic Publication: 2024 Jan 26 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1331363
Abstrakt:	Membrane vesicles (MVs) are envelope-derived extracellular sacs that perform a broad diversity of physiological functions in bacteria. While considerably studied in pathogenic microorganisms, the roles, relevance, and biotechnological potential of MVs from environmental bacteria are less well established. Acidithiobacillaceae family bacteria are active players in the sulfur and iron biogeochemical cycles in extremely acidic environments and drivers of the leaching of mineral ores contributing to acid rock/mine drainage (ARD/AMD) and industrial bioleaching. One key aspect of such a role is the ability of these bacteria to tightly interact with the mineral surfaces and extract electrons and nutrients to support their chemolithotrophic metabolism. Despite recent advances in the characterization of acidithiobacilli biofilms and extracellular matrix (ECM) components, our understanding of its architectural and mechanistic aspects remains scant. Using different microscopy techniques and nano-tracking analysis we show that vesiculation is a common phenomenon in distant members of the Acidithiobacillaceae family, and further explore the role of MVs in multicellular colonization behaviors using ' Fervidacidithiobacillus caldus ' as a bacterial model. Production of MVs in ' F. caldus ' occurred in both planktonic cultures and biofilms formed on sulfur surfaces, where MVs appeared individually or in chains resembling tube-shaped membranous structures (TSMSs) important for microbial communication. Liquid chromatography-mass spectrometry data and bioinformatic analysis of the MV-associated proteome revealed that ' F. caldus ' MVs were enriched in proteins involved in cell-cell and cell-surface processes and largely typified the MVs as outer MVs (OMVs). Finally, microbiological assays showed that amendment of ' F. caldus ' MVs to cells and/or biofilms affects collective colonizing behaviors relevant to the ecophysiology and applications of these acidophiles, providing grounds for their exploitation in biomining. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The handling editor AS declared a past co-authorship with the author MVe. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 Rossoni, Beard, Segura-Bidermann, Duarte-Ramírez, Osorio, Varas-Godoy, Martínez-Bellange, Vera, Quatrini and Castro.)
Databáze:	MEDLINE
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