Proximity Mapping of Desmosomes Reveals a Striking Shift in Their Molecular Neighborhood Associated With Maturation.

Autor:	Fülle JB; Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK., de Almeida RA; Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK., Lawless C; Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK., Stockdale L; Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK., Yanes B; Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK., Lane EB; Skin Research Institute of Singapore, Agency of Science Technology and Research (A∗STAR), Singapore, Singapore., Garrod DR; Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK. Electronic address: david.garrod@manchester.ac.uk., Ballestrem C; Wellcome Trust Centre for Cell-Matrix Research, University of Manchester, Manchester, UK. Electronic address: christoph.ballestrem@manchester.ac.uk.
Jazyk:	angličtina
Zdroj:	Molecular & cellular proteomics : MCP [Mol Cell Proteomics] 2024 Mar; Vol. 23 (3), pp. 100735. Date of Electronic Publication: 2024 Feb 10.
DOI:	10.1016/j.mcpro.2024.100735
Abstrakt:	Desmosomes are multiprotein adhesion complexes that link intermediate filaments to the plasma membrane, ensuring the mechanical integrity of cells across tissues, but how they participate in the wider signaling network to exert their full function is unclear. To investigate this, we carried out protein proximity mapping using biotinylation (BioID). The combined interactomes of the essential desmosomal proteins desmocollin 2a, plakoglobin, and plakophilin 2a (Pkp2a) in Madin-Darby canine kidney epithelial cells were mapped and their differences and commonalities characterized as desmosome matured from Ca 2+ dependence to the mature, Ca 2+ -independent, hyper-adhesive state, which predominates in tissues. Results suggest that individual desmosomal proteins have distinct roles in connecting to cellular signaling pathways and that these roles alter substantially when cells change their adhesion state. The data provide further support for a dualistic concept of desmosomes in which the properties of Pkp2a differ from those of the other, more stable proteins. This body of data provides an invaluable resource for the analysis of desmosome function. Competing Interests: Conflict of interest The authors declare no competing interests. (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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