| Autor: |
Yaglova NV; Laboratory of Endocrine System Development, A.P. Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution 'Petrovsky National Research Centre of Surgery', 119991 Moscow, Russia., Obernikhin SS; Laboratory of Endocrine System Development, A.P. Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution 'Petrovsky National Research Centre of Surgery', 119991 Moscow, Russia., Nazimova SV; Laboratory of Endocrine System Development, A.P. Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution 'Petrovsky National Research Centre of Surgery', 119991 Moscow, Russia., Tsomartova DA; Laboratory of Endocrine System Development, A.P. Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution 'Petrovsky National Research Centre of Surgery', 119991 Moscow, Russia.; Department of Human Anatomy and Histology, Federal State Funded Educational Unlike the Control Institution of Higher Education, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia., Timokhina EP; Laboratory of Endocrine System Development, A.P. Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution 'Petrovsky National Research Centre of Surgery', 119991 Moscow, Russia., Yaglov VV; Laboratory of Endocrine System Development, A.P. Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution 'Petrovsky National Research Centre of Surgery', 119991 Moscow, Russia., Tsomartova ES; Laboratory of Endocrine System Development, A.P. Avtsyn Research Institute of Human Morphology of Federal State Budgetary Scientific Institution 'Petrovsky National Research Centre of Surgery', 119991 Moscow, Russia.; Department of Human Anatomy and Histology, Federal State Funded Educational Unlike the Control Institution of Higher Education, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia., Chereshneva EV; Department of Human Anatomy and Histology, Federal State Funded Educational Unlike the Control Institution of Higher Education, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia., Ivanova MY; Department of Human Anatomy and Histology, Federal State Funded Educational Unlike the Control Institution of Higher Education, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia., Lomanovskaya TA; Department of Human Anatomy and Histology, Federal State Funded Educational Unlike the Control Institution of Higher Education, I.M. Sechenov First Moscow State Medical University, 119435 Moscow, Russia. |
| Abstrakt: |
Dichlorodiphenyltrichloroethane (DDT) is a wide-spread systemic pollutant with endocrine disrupting properties. Prenatal exposure to low doses of DDT has been shown to affect adrenal medulla growth and function. The role of postnatal exposure to DDT in developmental disorders remains unclear. The aim of the present investigation is to assess growth parameters and the expression of factors mediating the function and renewal of chromaffin cells in the adult adrenal medulla of male Wistar rats exposed to the endocrine disruptor o,p'-DDT since birth until sexual maturation. The DDT-exposed rats exhibited normal growth of the adrenal medulla but significantly decreased tyrosine hydroxylase production by chromaffin cells during postnatal period. Unlike the control, the exposed rats showed enhanced proliferation and reduced expression of nuclear β-catenin, transcription factor Oct4, and ligand of Sonic hedgehog after termination of the adrenal growth period. No expression of pluripotency marker Sox2 and absence of Ascl 1-positive progenitors were found in the adrenal medulla during postnatal ontogeny of the exposed and the control rats. The present findings indicate that an increase in proliferative activity and inhibition of the formation of reserve for chromaffin cell renewal, two main mechanisms for cell maintenance in adrenal medulla, in the adult DDT-exposed rats may reflect a compensatory reaction aimed at the restoration of catecholamine production levels. The increased proliferation of chromaffin cells in adults suggests excessive growth of the adrenal medulla. Thus, postnatal exposure to DDT alters cell physiology and increases the risk of functional insufficiency and hyperplasia of the adrenal medulla. |
