Optimizing the enzymatic production of biolubricants by the Taguchi method: Esterification of the free fatty acids from castor oil with 2-ethyl-1-hexanol catalyzed by Eversa Transform 2.0.

Autor: Monteiro RRC; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, 60455760 Fortaleza, Brazil., de Melo Neta MMF; Departamento de Engenharia Mecânica, Universidade Federal do Ceará, Campus do Pici, 60455760 Fortaleza, Brazil., Rocha WS; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, 60455760 Fortaleza, Brazil., Soares JB; Departamento de Engenharia de Transportes, Universidade Federal do Ceará, Campus do Pici, 60455760 Fortaleza, Brazil., de Luna FMT; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, 60455760 Fortaleza, Brazil; Departamento de Engenharia Mecânica, Universidade Federal do Ceará, Campus do Pici, 60455760 Fortaleza, Brazil., Fernandez-Lafuente R; Departamento de Biocatálisis, ICP-CSIC, Campus UAM-CSIC, 28049 Madrid, Spain. Electronic address: rfl@icp.csic.es., Vieira RS; Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, 60455760 Fortaleza, Brazil. Electronic address: rodrigo@gpsa.ufc.br.
Jazyk: angličtina
Zdroj: Enzyme and microbial technology [Enzyme Microb Technol] 2024 Apr; Vol. 175, pp. 110409. Date of Electronic Publication: 2024 Feb 05.
DOI: 10.1016/j.enzmictec.2024.110409
Abstrakt: The solvent-free esterification of the free fatty acids (FFAs) obtained by the hydrolysis of castor oil (a non-edible vegetable oil) with 2-ethyl-1-hexanol (a branched fatty alcohol) was catalyzed by different free lipases. Eversa Transform 2.0 (ETL) features surpassed most commercial lipases. Some process parameters were optimized by the Taguchi method (L16'). As a result, a conversion over 95% of the FFAs of castor oil into esters with lubricants properties was achieved under optimized reaction conditions (15 wt% of biocatalyst content, 1:4 molar ratio (FFAs/alcohol), 30 °C, 180 rpm, 96 h). The substrates molar ratio had the highest influence on the dependent variable (conversion at 24 h). FFAs/2-ethyl-1-hexanol esters were characterized regarding the physicochemical and tribological properties. Interestingly, the modification of the FFAs with 2-ethyl-1-hexanol by ETL increased the oxidative stability of the FFAs feedstock from 0.18 h to 16.83 h. The biolubricants presented a lower friction coefficient than the reference commercial mineral lubricant (0.052 ± 0.07 against 0.078 ± 0.04). Under these conditions, ETL catalyzed the oligomerization of ricinoleic acid (a hydroxyl fatty acid) into estolides, reaching a conversion of 25.15% of the initial FFAs (for the first time).
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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