| Autor: |
Matsui K; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Sekine H; Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, Tokyo 162-0056, Japan., Ishikawa J; Division for Advanced Medical Sciences, National Center for Child Health and Development, Tokyo 157-8535, Japan., Enosawa S; Division for Advanced Medical Sciences, National Center for Child Health and Development, Tokyo 157-8535, Japan.; Department of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Matsumoto N; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Inage Y; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.; Department of Pediatrics, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Kinoshita Y; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.; Department of Urology, Graduate School of Medicine, The University of Tokyo, Tokyo 113-8654, Japan., Morimoto K; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Yamamoto S; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan.; Department of Urology, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Koda N; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Yamanaka S; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Yokoo T; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan., Kobayashi E; Department of Kidney Regenerative Medicine, The Jikei University School of Medicine, Tokyo 105-8461, Japan. |
| Abstrakt: |
Human pluripotent stem cells have been employed in generating organoids, yet their immaturity compared to fetal organs and the limited induction of all constituent cell types remain challenges. Porcine fetal progenitor cells have emerged as promising candidates for co-culturing with human progenitor cells in regeneration and xenotransplantation research. This study focused on identifying proper preservation methods for porcine fetal kidneys, hearts, and livers, aiming to optimize their potential as cell sources. Extracted from fetal microminiature pigs, these organs were dissociated before and after cryopreservation-thawing, with subsequent cell quality evaluations. Kidney cells, dissociated and aggregated after vitrification in a whole-organ form, were successfully differentiated into glomeruli and tubules in vivo. In contrast, freezing hearts and livers before dissociation yielded suboptimal results. Heart cells, frozen after dissociation, exhibited pulsating heart muscle cells similar to non-frozen hearts. As for liver cells, we developed a direct tissue perfusion technique and successfully obtained highly viable liver parenchymal cells. Freezing dissociated liver cells, although inferior to their non-frozen counterparts, maintained the ability for colony formation. The findings of this study provide valuable insights into suitable preservation methods for porcine fetal cells from kidneys, hearts, and livers, contributing to the advancement of regeneration and xenotransplantation research. |
