Functionalized magnesium alloys obtained by superplastic forming process retain osteoinductive and antibacterial properties: An in-vitro study.

Autor: Tatullo M; Department of Translational Biomedicine and Neuroscience, School of Medicine, University of Bari 'Aldo Moro', 70124 Bari, Italy. Electronic address: marco.tatullo@uniba.it., Piattelli A; School of Dentistry, Saint Camillus International University of Health and Medical Sciences, 00131 Rome, Italy., Ruggiero R; Stem Cells and Medical Genetics Units, Tecnologica Research Institute and Marrelli Health, 88900 Crotone, Italy., Marano RM; Stem Cells and Medical Genetics Units, Tecnologica Research Institute and Marrelli Health, 88900 Crotone, Italy., Iaculli F; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, via S. Pansini 5, 80131 Naples, Italy., Rengo C; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, via S. Pansini 5, 80131 Naples, Italy., Papallo I; CeSMA, University of Naples Federico II, Corso Nicolangelo Protopisani, 80146 Naples, Italy., Palumbo G; Department of Mechanics, Polytechnic University of Bari, 70124 Bari, Italy., Chiesa R; Department of Chemistry, Materials and Chemical Engineering 'G. Natta', Politecnico di Milano, 20135 Milan, Italy., Paduano F; Stem Cells and Medical Genetics Units, Tecnologica Research Institute and Marrelli Health, 88900 Crotone, Italy., Spagnuolo G; Department of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples Federico II, via S. Pansini 5, 80131 Naples, Italy. Electronic address: gspagnuo@unina.it.
Jazyk: angličtina
Zdroj: Dental materials : official publication of the Academy of Dental Materials [Dent Mater] 2024 Mar; Vol. 40 (3), pp. 557-562. Date of Electronic Publication: 2024 Feb 07.
DOI: 10.1016/j.dental.2024.01.005
Abstrakt: Objectives: This study aimed to investigate the biocompatibility, osteogenic and antibacterial activity of biomedical devices based on Magnesium (Mg) Alloys manufactured by Superplastic Forming process (SPF) and subjected to Hydrothermal (HT) and Sol-Gel Treatment (Sol-Gel).
Methods: Mg-SPF devices subjected to Hydrothermal (Mg-SPF+HT) and Sol-Gel Treatment (Mg-SPF+Sol-Gel) were investigated. The biocompatibility of Mg-SPF+Sol-Gel and Mg-SPF+HT devices was observed by indirect and direct cytotoxicity assays, whereas the colonization of sample surfaces was assessed by confocal microscopy. qRT-PCR analysis and microbial growth curve analyses were employed to evaluate the osteogenic and antibacterial activity of both SPF-Mg treated devices, respectively.
Results: Mg-SPF+HT and Mg-SPF+Sol-Gel showed a high degree of biocompatibility. Analysis of mRNA expression of osteogenic genes in cells cultured on Mg-treated devices revealed a significant upregulation of the expression levels of BMP2 and Runx-2. Furthermore, the bacterial growth in strains developed in contact with both the Mg-SPF+HT and Mg-SPF+Sol-Gel devices was lower than that observed in the control.
Significance: Hydrothermal and Sol-Gel Treatments of Mg alloys obtained through the SPF process demonstrated bioactive, osteogenic and antibacterial activity, offering a promising alternative to conventional Mg-based devices. The obtained Mg-based materials may have the potential to enhance the tunability of temporary devices in maxillary reconstruction, eliminating the need for second surgeries, and ensuring a good bone reconstruction and a reduced implant failure rate due to bacterial infections.
Competing Interests: Declaration of Competing Interest The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.
(Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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