Enterobacteria in anaerobic digestion of dairy cattle wastewater: Assessing virulence and resistance for one health security.

Autor: Menezes KV; Graduate Program in Veterinary Sciences, Center for Agricultural Sciences and Engineering - CCAE, Federal University of Espírito Santo - UFES, Alto Universitário, no number, Guararema, Alegre, ES 29500-000, Brazil., Duarte CES; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES 29500-000, Brazil., Moreira MG; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES 29500-000, Brazil., Moreno TJC; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES 29500-000, Brazil., Pereira VJDS; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES 29500-000, Brazil., Ucella-Filho JGM; Department of Forestry and Wood Sciences, Federal University of Espírito Santo - UFES, Jerônimo Monteiro, ES 29550-000, Brazil., Otenio MH; Embrapa Dairy Cattle - Brazilian Agricultural Research Corporation, Juiz de Fora, Brazil., Ignacchiti MDC; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES 29500-000, Brazil., Resende JA; Graduate Program in Veterinary Sciences, Center for Agricultural Sciences and Engineering - CCAE, Federal University of Espírito Santo - UFES, Alto Universitário, no number, Guararema, Alegre, ES 29500-000, Brazil; Department of Pharmacy and Nutrition, Federal University of Espírito Santo - UFES, Alegre, ES 29500-000, Brazil. Electronic address: juliana.resende@ufes.br.
Jazyk: angličtina
Zdroj: Water research [Water Res] 2024 Mar 15; Vol. 252, pp. 121192. Date of Electronic Publication: 2024 Jan 24.
DOI: 10.1016/j.watres.2024.121192
Abstrakt: Samples from a dairy cattle waste-fed anaerobic digester were collected across seasons to assess sanitary safety for biofertilizer use. Isolated enterobacteria (suggestive of Escherichia coli) were tested for susceptibility to biocides, antimicrobials, and biofilm-forming capability. Results revealed a decrease in total bacteria, coliforms, and enterobacteria in biofertilizer compared to the effluent. Among 488 isolates, 98.12 % exhibited high biofilm formation. Biofertilizer isolates exhibited a similar biofilm formation capability as effluent isolates in summer, but greater propensity in winter. Resistance to biocides and antimicrobials varied, with tetracycline resistance reaching 19 %. Of the isolates, 25 were multidrug-resistant (MDR), with 64 % resistant to three drugs. Positive correlations were observed between MDR and increased biofilm formation capacity in both samples, while there was negative correlation between MDR and increased biocide resistance. A higher number of MDR bacteria were found in biofertilizer compared to the effluent, revealing the persistence of E. coli resistance, posing challenges to food safety and public health.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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