Anti-CRISPR Anopheles mosquitoes inhibit gene drive spread under challenging behavioural conditions in large cages.

Autor: D'Amato R; Genetics and Ecology Research Centre, Polo of Genomics, Genetics and Biology (Polo GGB), Terni, Italy., Taxiarchi C; Department of Life Sciences, Imperial College London, London, UK., Galardini M; Biological Design Center, Boston University, Boston, MA, USA.; Institute for Molecular Bacteriology, TWINCORE Centre for Experimental and Clinical Infection Research, a joint venture between the Hannover Medical School (MHH) and the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.; Cluster of Excellence RESIST (EXC 2155), Hannover Medical School (MHH), Hannover, Germany., Trusso A; Genetics and Ecology Research Centre, Polo of Genomics, Genetics and Biology (Polo GGB), Terni, Italy., Minuz RL; Genetics and Ecology Research Centre, Polo of Genomics, Genetics and Biology (Polo GGB), Terni, Italy., Grilli S; Department of Life Sciences, Imperial College London, London, UK., Somerville AGT; Department of Life Sciences, Imperial College London, London, UK., Shittu D; Department of Life Sciences, Imperial College London, London, UK., Khalil AS; Biological Design Center, Boston University, Boston, MA, USA.; Department of Biomedical Engineering, Boston University, Boston, MA, USA.; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA., Galizi R; Centre for Applied Entomology and Parasitology, School of Life Sciences, Keele University, Keele, UK., Crisanti A; Department of Life Sciences, Imperial College London, London, UK.; Department of Molecular Medicine, University of Padova, Padua, Italy., Simoni A; Genetics and Ecology Research Centre, Polo of Genomics, Genetics and Biology (Polo GGB), Terni, Italy. a.simoni@imperial.ac.uk.; Department of Life Sciences, Imperial College London, London, UK. a.simoni@imperial.ac.uk., Müller R; Genetics and Ecology Research Centre, Polo of Genomics, Genetics and Biology (Polo GGB), Terni, Italy. r.muller@itg.be.; Unit of Entomology, Department of Biomedical Sciences, Institute of Tropical Medicine, Antwerp, Belgium. r.muller@itg.be.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Feb 01; Vol. 15 (1), pp. 952. Date of Electronic Publication: 2024 Feb 01.
DOI: 10.1038/s41467-024-44907-x
Abstrakt: CRISPR-based gene drives have the potential to spread within populations and are considered as promising vector control tools. A doublesex-targeting gene drive was able to suppress laboratory Anopheles mosquito populations in small and large cages, and it is considered for field application. Challenges related to the field-use of gene drives and the evolving regulatory framework suggest that systems able to modulate or revert the action of gene drives, could be part of post-release risk-mitigation plans. In this study, we challenge an AcrIIA4-based anti-drive to inhibit gene drive spread in age-structured Anopheles gambiae population under complex feeding and behavioural conditions. A stochastic model predicts the experimentally-observed genotype dynamics in age-structured populations in medium-sized cages and highlights the necessity of large-sized cage trials. These experiments and experimental-modelling framework demonstrate the effectiveness of the anti-drive in different scenarios, providing further corroboration for its use in controlling the spread of gene drive in Anopheles.
(© 2024. The Author(s).)
Databáze: MEDLINE
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