Improving the quality of quantitative polymerase chain reaction experiments: 15 years of MIQE.

Autor: Bustin SA; Medical Technology Research Centre, Anglia Ruskin University, Chelmsford, Essex, CM1 1SQ, UK. Electronic address: stephen.bustin@aru.ac.uk.
Jazyk: angličtina
Zdroj: Molecular aspects of medicine [Mol Aspects Med] 2024 Apr; Vol. 96, pp. 101249. Date of Electronic Publication: 2024 Jan 29.
DOI: 10.1016/j.mam.2024.101249
Abstrakt: The quantitative polymerase chain reaction (qPCR) is fundamental to molecular biology. It is not just a laboratory technique, qPCR is a bridge between research and clinical practice. Its theoretical foundations guide the design of experiments, while its practical implications extend to diagnostics, treatment, and research advancements in the life sciences, human and veterinary medicine, agriculture, and forensics. However, the accuracy, reliability and reproducibility of qPCR data face challenges arising from various factors associated with experimental design, execution, data analysis and inadequate reporting details. Addressing these concerns, the Minimum Information for the Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines have emerged as a cohesive framework offering a standardised set of recommendations that describe the essential information required for assessing qPCR experiments. By emphasising the importance of methodological rigour, the MIQE guidelines have made a major contribution to improving the trustworthiness, consistency, and transparency of many published qPCR results. However, major challenges related to awareness, resources, and publication pressures continue to affect their consistent application.
(Copyright © 2024 The Author. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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