The Impact of Activating Agents on Non-Enzymatic Nucleic Acid Extension Reactions.

Autor: Callaghan KL; Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria, 3010, Australia., Sherrell PC; Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria, 3010, Australia.; School of Science, RMIT University, Melbourne, Victoria, 3000, Australia., Ellis AV; Department of Chemical Engineering, The University of Melbourne, Parkville, Victoria, 3010, Australia.
Jazyk: angličtina
Zdroj: Chembiochem : a European journal of chemical biology [Chembiochem] 2024 Apr 02; Vol. 25 (7), pp. e202300859. Date of Electronic Publication: 2024 Feb 12.
DOI: 10.1002/cbic.202300859
Abstrakt: Non-enzymatic template-directed primer extension is increasingly being studied for the production of RNA and DNA. These reactions benefit from producing RNA or DNA in an aqueous, protecting group free system, without the need for expensive enzymes. However, these primer extension reactions suffer from a lack of fidelity, low reaction rates, low overall yields, and short primer extension lengths. This review outlines a detailed mechanistic pathway for non-enzymatic template-directed primer extension and presents a review of the thermodynamic driving forces involved in entropic templating. Through the lens of entropic templating, the rate and fidelity of a reaction are shown to be intrinsically linked to the reactivity of the activating agent used. Thus, a strategy is discussed for the optimization of non-enzymatic template-directed primer extension, providing a path towards cost-effective in vitro synthesis of RNA and DNA.
(© 2024 The Authors. ChemBioChem published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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