In vitro and in vivo toxicity of thiolated and PEGylated organosilica nanoparticles.

Autor: Zhaisanbayeva BA; School of Engineering and Digital Science, Nazarbayev University, 010000 Astana, Kazakhstan; School of Sciences and Humanities, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: balnur.zhaisanbayeva@nu.edu.kz., Mun EA; School of Sciences and Humanities, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: ellina.mun@nu.edu.kz., Ulmanova L; School of Sciences and Humanities, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: leila.ulmanova@alumni.nu.edu.kz., Zhunissova Z; School of Sciences and Humanities, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: zarina.zhunissova@alumni.nu.edu.kz., Umbayev B; National Laboratory Astana, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: bauyrzhan.umbayev@nu.edu.kz., Olzhayev F; National Laboratory Astana, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: folzhayev@nu.edu.kz., Vorobjev IA; School of Sciences and Humanities, Nazarbayev University, 010000 Astana, Kazakhstan; National Laboratory Astana, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: ivan.vorobyev@nu.edu.kz., Hortelano G; School of Sciences and Humanities, Nazarbayev University, 010000 Astana, Kazakhstan. Electronic address: gonzalo.hortelano@nu.edu.kz., Khutoryanskiy VV; School of Pharmacy, University of Reading, Reading, UK. Electronic address: v.khutoryanskiy@reading.ac.uk.
Jazyk: angličtina
Zdroj: International journal of pharmaceutics [Int J Pharm] 2024 Mar 05; Vol. 652, pp. 123852. Date of Electronic Publication: 2024 Jan 26.
DOI: 10.1016/j.ijpharm.2024.123852
Abstrakt: This study comprises the comprehensive toxicological assessment of thiolated organosilica nanoparticles (NPs) synthesised from 3-mercaptopropyltrimethoxysilane (MPTS). We investigated the influence of three different types of nanoparticles synthesised from 3-mercaptopropyltrimethoxysilane: the starting thiolated silica (Si-NP-SH) and their derivatives prepared by surface PEGylation with PEG 750 (Si-NP-PEG750) and 5000 Da (Si-NP-PEG5000) on biological subjects from in vitro to in vivo experiments to explore the possible applications of those nanoparticles in biomedical research. As a result of this study, we generated a comprehensive understanding of the toxicological properties of these nanoparticles, including their cytotoxicity in different cell lines, hemolytic properties, in vitro localisation, mucosal irritation properties and biodistribution in BALB/c mice. Our findings indicate that all three types of nanoparticles can be considered safe and have promising prospects for use in biomedical applications. Nanoparticles did not affect the viability of HPF, MCF7, HEK293 and A549 cell lines at low concentrations (up to 100 µg/mL); moreover, they did not cause organ damage to BALB/c mice at concentrations of 10 mg/kg. The outcomes of this study enhance our understanding of the impact of organosilica nanoparticles on health and the environment, which is vital for developing silica nanoparticle-based drug delivery systems and provides opportunities to expand the applications of organosilica nanoparticles.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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