| Autor: |
Ruparelia KC; Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK., Zeka K; Zayed Centre for Research into Rare Disease in Children, University College London, London WC1E 6BT, UK., Beresford KJM; Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK., Wilsher NE; Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK., Potter GA; Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK., Androutsopoulos VP; Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK., Brucoli F; Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK., Arroo RRJ; Leicester School of Pharmacy, De Montfort University, The Gateway, Leicester LE1 9BH, UK. |
| Abstrakt: |
Naturally occurring stilbenoids, such as the ( E )-stilbenoid resveratrol and the ( Z )-stilbenoid combretastatin A4, have been considered as promising lead compounds for the development of anticancer drugs. The antitumour properties of stilbenoids are known to be modulated by cytochrome P450 enzymes CYP1A1 and CYP1B1, which contribute to extrahepatic phase I xenobiotic and drug metabolism. Thirty-four methyl ether analogues of resveratrol were synthesised, and their anticancer properties were assessed, using the MTT cell proliferation assay on a panel of human breast cell lines. Breast tumour cell lines that express CYP1 were significantly more strongly affected by the resveratrol analogues than the cell lines that did not have CYP1 activity. Metabolism studies using isolated CYP1 enzymes provided further evidence that ( E )-stilbenoids can be substrates for these enzymes. Structures of metabolic products were confirmed by comparison with synthetic standards and LC-MS co-elution studies. The most promising stilbenoid was ( E )-4,3',4',5'-tetramethoxystilbene (DMU212). The compound itself showed low to moderate cytotoxicity, but upon CYP1-catalysed dealkylation, some highly cytotoxic metabolites were formed. Thus, DMU212 selectively affects proliferation of cells that express CYP1 enzymes. |
