Recent Updates Regarding the Antiproliferative Activity of Galium verum Extracts on A375 Human Malignant Melanoma Cell Line.

Autor: Semenescu AD; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Moacă EA; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Iftode A; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Dehelean CA; Department of Toxicology, Drug Industry, Management and Legislation, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Research Centre for Pharmaco-Toxicological Evaluation, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Tchiakpe-Antal DS; Department of Pharmaceutical Botany, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Vlase L; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, 'Iuliu Hatieganu' University of Medicine and Pharmacy, 8th Victor Babes Street, 400347 Cluj-Napoca, Romania., Rotunjanu S; Department of Pharmacology-Pharmacotherapy, Faculty of Pharmacy, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Muntean D; Department of Microbiology, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania.; Multidisciplinary Research Center on Antimicrobial Resistance, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Chiriac SD; Department X-Surgery II, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timisoara, Romania., Chioibaş R; Department IX-Surgery I, Faculty of Medicine, 'Victor Babes' University of Medicine and Pharmacy Timisoara, 2nd Eftimie Murgu Square, 300041 Timișoara, Romania.; CBS Medcom Hospital, 12th Popa Sapca Street, 300047 Timisoara, Romania.
Jazyk: angličtina
Zdroj: Life (Basel, Switzerland) [Life (Basel)] 2024 Jan 11; Vol. 14 (1). Date of Electronic Publication: 2024 Jan 11.
DOI: 10.3390/life14010112
Abstrakt: The biological activity of Galium verum herba was exerted on various tumor cell lines with incredible results, but their potential effect on malignant melanoma has not been established yet. Therefore, the current study was structured in two directions: (i) the investigation of the phytochemical profile of diethyl ether (GvDEE) and butanol (GvBuOH) extracts of G. verum L. and (ii) the evaluation of their biological profile on A375 human malignant melanoma cell line. The GvDEE extract showed an FT-IR profile different from the butanol one, with high antioxidant capacity (EC 50 of GvDEE = 0.12 ± 0.03 mg/mL > EC 50 of GvBuOH = 0.18 ± 0.05 mg/mL). The GvDEE extract also showed antimicrobial potential, especially against Gram-positive bacteria strains, compared to the butanol extract, which has no antimicrobial activity against any bacterial strain tested. The results regarding the antitumor potential showed that both extracts decreased A375 cell viability largely (69% at a dose of 55 µg/mL of the GvDEE extract). Moreover, both extracts induce nuclear fragmentation by forming apoptotic bodies and slight chromatin condensation, which is more intense for GvDEE. Considering the results, one can state that the Galium verum herba possesses antitumor effects on the A375 human malignant melanoma cell line, a promising phytocompound for the antitumor approach to skin cancer.
Databáze: MEDLINE
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