| Autor: |
Deyneko DV; Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia.; Laboratory of Arctic Mineralogy and Material Sciences, Kola Science Centre RAS, 14 Fersman Str., 184209 Apatity, Russia., Lebedev VN; Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia., Barbaro K; Istituto Zooprofilattico Sperimentale Lazio e Toscana 'M. Aleandri', Via Appia Nuova 1411, 00178 Rome, Italy., Titkov VV; Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia., Lazoryak BI; Chemistry Department, Lomonosov Moscow State University, Leninskie Gory 1, 119991 Moscow, Russia., Fadeeva IV; A.A. Baikov Institute of Metallurgy and Material Science, Russian Academy of Sciences, Leninsky Prospect 49, 119334 Moscow, Russia., Gosteva AN; Tananaev Institute of Chemistry, Kola Science Centre RAS, Akademgorodok 26A, 184209 Apatity, Russia., Udyanskaya IL; Department of Analytical, Physical and Colloid Chemistry, Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119048 Moscow, Russia., Aksenov SM; Laboratory of Arctic Mineralogy and Material Sciences, Kola Science Centre RAS, 14 Fersman Str., 184209 Apatity, Russia.; Geological Institute, Kola Science Centre, Russian Academy of Sciences, 14 Fersman Street, 184209 Apatity, Russia., Rau JV; Department of Analytical, Physical and Colloid Chemistry, Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University, Trubetskaya 8, Build. 2, 119048 Moscow, Russia.; Istituto di Struttura della Materia, Consiglio Nazionale delle Ricerche (ISM-CNR), Via del Fosso del Cavaliere 100, 00133 Rome, Italy. |
| Abstrakt: |
β-Tricalcium phosphate (β-TCP) is widely used as bone implant material. It has been observed that doping the β-TCP structure with certain cations can help in combating bacteria and pathogenic microorganisms. Previous literature investigations have focused on tricalcium phosphate structures with silver, copper, zinc, and iron cations. However, there are limited studies available on the biological properties of β-TCP containing nickel and cobalt ions. In this work, Ca 10.5- x Ni x (PO 4 ) 7 and Ca 10.5- x Co x (PO 4 ) 7 solid solutions with the β-Ca 3 (PO 4 ) 2 structure were synthesized by a high-temperature solid-state reaction. Structural studies revealed the β-TCP structure becomes saturated at 9.5 mol/% for Co 2+ or Ni 2+ ions. Beyond this saturation point, Ni 2+ and Co 2+ ions form impurity phases after complete occupying of the octahedral M5 site. The incorporation of these ions into the β-TCP crystal structure delays the phase transition to the α-TCP phase and stabilizes the structure as the temperature increases. Biocompatibility tests conducted on adipose tissue-derived mesenchymal stem cells (aMSC) using the (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (MTT) assay showed that all prepared samples did not exhibit cytotoxic effects. Furthermore, there was no inhibition of cell differentiation into the osteogenic lineage. Antibacterial properties were studied on the C. albicans fungus and on E. coli , E. faecalis , S. aureus, and P. aeruginosa bacteria strains. The Ni- and Co-doped β-TCP series exhibited varying degrees of bacterial growth inhibition depending on the doping ion concentration and the specific bacteria strain or fungus. The combination of antibacterial activity and cell-friendly properties makes these phosphates promising candidates for anti-infection bone substitute materials. |
