High-intensity intermittent training ameliorates methotrexate-induced acute lung injury.

Autor: Rajizadeh MA; Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran.; Department of Physiology and Pharmacology, Kerman University of Medical Sciences, Kerman, Iran.; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran., Hosseini MH; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.; Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Bahrami M; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran.; Department of Exercise Physiology, Faculty of Physical Education and Sports Sciences, Shahid Bahonar University of Kerman, Kerman, Iran., Bahri F; Department of Clinical Biochemistry, Faculty of Medicine, Kerman University of Medical Sciences, Kerman, Iran., Rostamabadi F; Noncommunicable Diseases Research center, Bam university of medical sciences, Bam, Iran.; Department of Medical Immunology, Faculty of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran., Bagheri F; Pathology and Stem Cell Research Center, Department of Pathology, Afzalipour School of Medicine, Kerman, Iran., Khoramipour K; Department of Physiology and Pharmacology, Kerman University of Medical Sciences, Kerman, Iran., Najafipour H; Cardiovascular Research Center, Institute of Basic and Clinical physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran., Bejeshk MA; Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran. M.bejeshk@yahoo.com.; Department of Physiology and Pharmacology, Kerman University of Medical Sciences, Kerman, Iran. M.bejeshk@yahoo.com.; Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran. M.bejeshk@yahoo.com.
Jazyk: angličtina
Zdroj: BMC pulmonary medicine [BMC Pulm Med] 2024 Jan 20; Vol. 24 (1), pp. 45. Date of Electronic Publication: 2024 Jan 20.
DOI: 10.1186/s12890-024-02853-w
Abstrakt: Inflammation and oxidative stress are recognized as two primary causes of lung damage induced by methotrexate, a drug used in the treatment of cancer and immunological diseases. This drug triggers the generation of oxidants, leading to lung injury. Given the antioxidant and anti-inflammatory effects of high-intensity intermittent training (HIIT), our aim was to evaluate the therapeutic potential of HIIT in mitigating methotrexate-induced lung damage in rats. Seventy male Wistar rats were randomly divided into five groups: CTL (Control), HIIT (High-intensity intermittent training), ALI (Acute Lung Injury), HIIT+ALI (pretreated with HIIT), and ALI + HIIT (treated with HIIT).HIIT sessions were conducted for 8 weeks. At the end of the study, assessments were made on malondialdehyde, total antioxidant capacity (TAC), superoxide dismutase (SOD), glutathione peroxidase (Gpx), myeloperoxidase (MPO), interleukin 10 (IL-10), tumor necrosis factor-alpha (TNF-α), gene expression of T-bet, GATA3, FOXP3, lung wet/dry weight ratio, pulmonary capillary permeability, apoptosis (Caspase-3), and histopathological indices.Methotrexate administration resulted in increased levels of TNF-α, MPO, GATA3, caspase-3, and pulmonary edema indices, while reducing the levels of TAC, SOD, Gpx, IL-10, T-bet, and FOXP3. Pretreatment and treatment with HIIT reduced the levels of oxidant and inflammatory factors, pulmonary edema, and other histopathological indicators. Concurrently, HIIT increased the levels of antioxidant and anti-inflammatory factors.
(© 2024. The Author(s).)
Databáze: MEDLINE
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